Jakab Csaba, Rusvai Miklós, Gálfi Péter, Szabára Agnes, Szabó Zoltán, Kulka Janina
Department of Pathology and Forensic Veterinary Medicine, István u. 2 H-1078 Budapest, Hungary.
Acta Vet Hung. 2010 Dec;58(4):423-30. doi: 10.1556/AVet.58.2010.4.3.
Claudins are key tight junctional proteins between adjacent epithelial, mesothelial or endothelial cells, which are responsible for the permeability of the paracellular space. This paper describes that the endothelial cells of normal hepatic arterioles, portal venules and portal lymphatics as well as the endothelium of sinusoids from dogs show strong membranous claudin-5 cross-reactivity. In 25 liver biopsy samples taken from dogs with portal vein hypoperfusion, an increased number of arterioles was detected in the portal areas (PAs) by the use of humanised anti-claudin-5 antibody. The increased number of hyperplastic hepatic arterioles per PA was 5-6, 8-12 and 15-20 in the case of small, medium-sized and large PAs, respectively. It is suggested that the claudin-5 marker can improve the detection of hepatic arteriolar proliferation in the PAs of liver samples.
闭合蛋白是相邻上皮细胞、间皮细胞或内皮细胞之间关键的紧密连接蛋白，负责细胞旁间隙的通透性。本文描述了正常肝小动脉、门静脉小支和门静脉淋巴管的内皮细胞以及犬类肝血窦的内皮细胞显示出强烈的膜性闭合蛋白-5交叉反应性。在从门静脉灌注不足的犬类获取的25份肝活检样本中，通过使用人源化抗闭合蛋白-5抗体，在门静脉区域（PA）检测到小动脉数量增加。在小、中、大型PA中，每个PA增生性肝小动脉数量增加分别为5 - 6条、8 - 12条和15 - 20条。提示闭合蛋白-5标记物可改善肝样本PA中肝小动脉增殖的检测。
