Opalka Marek, Kugla-Owczarska Justyna, Kaminska Barbara, Puchajda-Skowronska Helena, Hryniewicka Wioletta, Dusza Luiza
Department of Animal Physiology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland.
Acta Vet Hung. 2008 Sep;56(3):379-91. doi: 10.1556/AVet.56.2008.3.11.
The aim of this study was to investigate how long-term feeding of ganders with diets containing different levels of phytoestrogens affects the morphometry of testes, plasma steroid levels, sperm parameters and reproductive performance. Male Bilgoraj geese were fed diets containing grass meal (with low phytoestrogen levels) and those containing alfalfa meal and soybean meal (with higher levels of phytoestrogens). Testes were obtained from ganders at the peak of mating and the ongoing laying period as well as at the beginning of photorefractoriness. The morphometric parameters were measured in frozen testicular sections. Blood was collected monthly from December to June, and plasma testosterone, androstenedione and corticosterone concentrations were determined. During the mating season, semen was collected and its quantity and quality were investigated. In the flocks (ganders with geese) reproductive output was noted. Seasonal changes were observed in the morphology of testes and in plasma testosterone, androstenedione and corticosterone concentrations in ganders. The feeding of ganders with dietary meals containing higher levels of phytoestrogens did not affect the plasma steroid contents or the majority of the morphometric parameters of testes, except for the height of the seminiferous epithelium, which was lowered. Moreover, diets with higher levels of phytoestrogens decreased the volume of ejaculates and the percentage of normal spermatozoa. However, these changes were not reflected in the fertilisation rates.
本研究的目的是调查长期给雄鹅饲喂含有不同水平植物雌激素的日粮如何影响睾丸形态测量、血浆类固醇水平、精子参数和繁殖性能。雄性比尔戈拉伊鹅被饲喂含有草粉（植物雌激素水平低）的日粮以及含有苜蓿粉和豆粕（植物雌激素水平较高）的日粮。在交配高峰期、产蛋期以及光不应期开始时从雄鹅获取睾丸。在冷冻的睾丸切片中测量形态学参数。从12月至6月每月采集血液，测定血浆睾酮、雄烯二酮和皮质酮浓度。在交配季节，采集精液并调查其数量和质量。在鹅群（雄鹅与雌鹅）中记录繁殖产量。观察到雄鹅睾丸形态以及血浆睾酮、雄烯二酮和皮质酮浓度的季节性变化。给雄鹅饲喂含有较高水平植物雌激素的日粮，除了生精上皮高度降低外，并未影响血浆类固醇含量或睾丸的大多数形态学参数。此外，含有较高水平植物雌激素的日粮减少了射精量和正常精子的百分比。然而，这些变化并未反映在受精率上。
