a Department of Animal Science , National Chung Hsing University , Taichung , Taiwan.
b Kaohsiung Animal Propagation Station , Livestock Research Institute, Council of Agriculture , Pingtung , Taiwan.
Anim Biotechnol. 2019 Jan;30(1):43-56. doi: 10.1080/10495398.2018.1432488. Epub 2018 Feb 9.
The purpose of this study was to investigate the change in protein expression in the testes of ganders at various breeding stages. A total of nine 3-year-old male White Roman ganders were used. The blood and testis samples were collected at the nonbreeding, sexual reactivation, and breeding stages for sex hormone analysis and proteomic analysis, respectively. The testicular weight and serum testosterone observed for ganders at the breeding stage were higher than those for ganders at nonbreeding and sexual reactivation stages (P < 0.05). There were 124 protein spots differentially expressed in the testes of ganders at various reproductive stages. A total of 107 protein spots of 74 proteins was identified through mass spectrometry. Most of the differentially expressed proteins were responsible for the molecular functions of protein binding (24%) and catalytic activity (16%). A functional pathway analysis suggested that proteins involved in steroidogenesis, metabolism, and spermatogenesis pathways changed in the White Roman geese at various reproductive stages. In conclusion, ganders at various reproductive stages exhibited different levels of testosterone and protein expression in the testes. The varied levels of the proteins might be essential and unique key factors in seasonal reproduction in ganders.
本研究旨在探讨不同繁殖阶段公鹅睾丸中蛋白质表达的变化。共使用了 9 只 3 岁雄性白罗曼公鹅。分别在非繁殖期、性再激活期和繁殖期采集血液和睾丸样本，用于性激素分析和蛋白质组分析。繁殖期公鹅的睾丸重量和血清睾丸酮水平高于非繁殖期和性再激活期公鹅（P<0.05）。在不同繁殖阶段的公鹅睾丸中，有 124 个蛋白质点表达差异。通过质谱鉴定出了 74 种蛋白质中的 107 个蛋白质点。大多数差异表达的蛋白质负责蛋白质结合（24%）和催化活性（16%）等分子功能。功能途径分析表明，在不同繁殖阶段的白罗曼鹅中，参与类固醇生成、代谢和精子发生途径的蛋白质发生了变化。总之，不同繁殖阶段的公鹅睾丸中表现出不同水平的睾丸酮和蛋白质表达。这些蛋白水平的变化可能是公鹅季节性繁殖的重要且独特的关键因素。
