催乳素抑制金鱼鳞片中的破骨细胞活性：催乳素在硬骨鱼中的一种新型直接作用。

Prolactin inhibits osteoclastic activity in the goldfish scale: a novel direct action of prolactin in teleosts.

作者信息

Takahashi Hideya, Suzuki Nobuo, Takagi Chiyo, Ikegame Mika, Yamamoto Toshio, Takahashi Akiyoshi, Moriyama Shunsuke, Hattori Atsuhiko, Sakamoto Tatsuya

机构信息

Ushimado Marine Laboratory, Graduate School of Natural Science and Technology, Okayama University, Setouchi, Okayama, Japan.

出版信息

Zoolog Sci. 2008 Jul;25(7):739-45. doi: 10.2108/zsj.25.739.

DOI:10.2108/zsj.25.739

PMID:18828661

Abstract

In teleosts, prolactin is involved in calcium regulation, but its role in scale/bone metabolism is unclear. Using the in-vitro system with goldfish scales developed recently, we explored the effects of teleost prolactin, growth hormone, and somatolactin on osteoclasts and osteoblasts. Addition of prolactin at concentrations of 0.01-100 ng/ml reduced osteoclastic activity, partly via osteoclast apoptosis, after 6-18 h incubation. Conversely, growth hormone and somatolactin at a concentration of 100 ng/ml increased osteoclastic activity after 18 h incubation, indicating the specificity of the inhibitory effect of prolactin on osteoclastic activity. On the other hand, these three hormones promoted osteoblastic activity at concentrations of 10-100 ng/ml. The results from this study are the first demonstration of direct effects of prolactin on scale/bone metabolism and osteoclastic activity in a teleost.

摘要

在硬骨鱼中，催乳素参与钙调节，但其在鳞片/骨骼代谢中的作用尚不清楚。利用最近开发的金鱼鳞片体外系统，我们探究了硬骨鱼催乳素、生长激素和生长抑素对破骨细胞和成骨细胞的影响。在孵育6-18小时后，添加浓度为0.01-100 ng/ml的催乳素可降低破骨细胞活性，部分是通过破骨细胞凋亡实现的。相反，浓度为100 ng/ml的生长激素和生长抑素在孵育18小时后会增加破骨细胞活性，这表明催乳素对破骨细胞活性的抑制作用具有特异性。另一方面，这三种激素在浓度为10-100 ng/ml时可促进成骨细胞活性。本研究结果首次证明了催乳素对硬骨鱼鳞片/骨骼代谢和破骨细胞活性具有直接影响。