Institute of Nature and Environmental Technology, Kanazawa University, Housu-gun, Ishikawa, Japan.
Bone. 2011 May 1;48(5):1186-93. doi: 10.1016/j.bone.2011.02.004. Epub 2011 Feb 17.
The effect of fugu parathyroid hormone 1 (fugu PTH1) on osteoblasts and osteoclasts in teleosts was examined with an assay system using teleost scale and the following markers: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Synthetic fugu PTH1 (1-34) (100pg/ml-10ng/ml) significantly increased ALP activity at 6h of incubation. High-dose (10ng/ml) fugu PTH1 significantly increased ALP activity even after 18h of incubation. In the case of TRAP activity, fugu PTH1 did not change at 6h of incubation, but fugu PTH1 (100pg/ml-10ng/ml) significantly increased TRAP activity at 18h. Similar results were obtained for human PTH (1-34), but there was an even greater response with fugu PTH1 than with human PTH. In vitro, we demonstrated that both the receptor activator of the NF-κB ligand in osteoblasts and the receptor activator NF-κB mRNA expression in osteoclasts increased significantly by fugu PTH1 treatment. In an in vivo experiment, fugu PTH1 induced hypercalcemia resulted from the increase of both osteoblastic and osteoclastic activities in the scale as well as the decrease of scale calcium contents after fugu PTH1 injection. In addition, an in vitro experiment with intramuscular autotransplanted scale indicated that the ratio of multinucleated osteoclasts/mononucleated osteoclasts in PTH-treated scales was significantly higher than that in the control scales. Thus, we concluded that PTH acts on osteoblasts and osteoclasts in the scales and regulates calcium metabolism in goldfish.
我们采用鱼类鳞片测定系统以及碱性磷酸酶（ALP）和抗酒石酸酸性磷酸酶（TRAP）这两种标志物，研究了河豚甲状旁腺素 1（fugu PTH1）对硬骨鱼类成骨细胞和破骨细胞的作用。结果显示，合成的河豚 PTH1（1-34）（100pg/ml-10ng/ml）可在孵育 6h 时显著增加 ALP 活性，高剂量（10ng/ml）河豚 PTH1 甚至在孵育 18h 后仍能显著增加 ALP 活性。至于 TRAP 活性，孵育 6h 时河豚 PTH1 并未发生变化，但河豚 PTH1（100pg/ml-10ng/ml）可在孵育 18h 时显著增加 TRAP 活性。类似的结果也见于人 PTH（1-34），但河豚 PTH1 的反应比人 PTH 更强烈。体外实验表明，河豚 PTH1 可显著增加成骨细胞中 NF-κB 配体受体激活剂和破骨细胞中 NF-κB 受体激活剂 mRNA 的表达。在体内实验中，河豚 PTH1 可诱导草鱼发生高钙血症，其原因是注射河豚 PTH1 后，鱼鳞中成骨细胞和破骨细胞活性增加，鱼鳞中钙含量降低。此外，肌肉内自体移植鱼鳞的体外实验表明，经 PTH 处理的鱼鳞中多核破骨细胞/单核破骨细胞的比值显著高于对照组。综上，我们得出结论：PTH 可作用于鱼鳞中的成骨细胞和破骨细胞，调节金鱼的钙代谢。
