Wang Li, Pan Qingshan, Xue Qin, Cui Jun, Qi Changming
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agriculture University, No. 2 Yuanming Yuan Xi Lu, Beijing 100094, China.
Am J Vet Res. 2008 Oct;69(10):1341-5. doi: 10.2460/ajvr.69.10.1341.
To evaluate the changes in concentrations of matrix metalloproteinase (MMP)-2 and MMP-9 in the precorneal tear film of dogs with Pseudomonas aeruginosa-associated keratitis during corneal healing and stromal remodeling.
10 dogs with unilateral P aeruginosa-associated keratitis and 10 clinically normal dogs.
Precorneal tear film samples were collected from both eyes of 10 dogs with unilateral P aeruginosa-associated keratitis on the day of admission to the hospital and then at various time points until complete healing of the cornea was achieved. Precorneal tear film samples were also collected from both eyes of 10 clinically normal adult dogs (control group). Concentrations of MMP-2 and MMP-9 in precorneal tear film samples from each group were determined via gelatin zymography for comparison.
The proteolytic processes in the ulcerated eyes decreased as corneal healing progressed. On the day of admission, concentrations of latent and active forms of MMP-2 and MMP-9 in ulcerated eyes were significantly higher than values in the contralateral unaffected eyes in dogs with P aeruginosa-associated keratitis; concentrations of latent MMP-2 and MMP-9 were also greater than control group values. Concentrations of latent and active forms of MMP-2 and MMP-9 in the healed eyes of dogs with P aeruginosa-associated keratitis were significantly lower than concentrations in the ulcerated eyes on the day of admission.
Results suggest that reduction of precorneal tear film concentrations of MMPs by use of proteinase inhibitors may be effective in the treatment of dogs with P aeruginosa-associated keratitis.
评估铜绿假单胞菌相关性角膜炎犬角膜愈合和基质重塑过程中角膜前泪膜中基质金属蛋白酶（MMP）-2和MMP-9浓度的变化。
10只单侧铜绿假单胞菌相关性角膜炎犬和10只临床正常犬。
对10只单侧铜绿假单胞菌相关性角膜炎犬入院当天及其后各个时间点直至角膜完全愈合时，采集双眼的角膜前泪膜样本。还从10只临床正常成年犬（对照组）的双眼采集角膜前泪膜样本。通过明胶酶谱法测定每组角膜前泪膜样本中MMP-2和MMP-9的浓度并进行比较。
随着角膜愈合进程，溃疡眼的蛋白水解过程减弱。入院当天，铜绿假单胞菌相关性角膜炎犬溃疡眼中潜伏型和活性型MMP-2和MMP-9的浓度显著高于对侧未受影响眼的值；潜伏型MMP-2和MMP-9的浓度也高于对照组值。铜绿假单胞菌相关性角膜炎犬愈合眼中潜伏型和活性型MMP-2和MMP-9的浓度显著低于入院当天溃疡眼中的浓度。
结果表明，使用蛋白酶抑制剂降低角膜前泪膜中MMPs的浓度可能对治疗铜绿假单胞菌相关性角膜炎犬有效。
