Singh Arti, Maurya O P S, Jagannadhan M V, Patel Ashok
Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh India.
J Ocul Biol Dis Infor. 2012 Dec 29;5(2):31-5. doi: 10.1007/s12177-012-9096-8. eCollection 2012.
The purpose of this paper is to determine the active form of matrix metalloproteinases (MMP-2 and MMP-9) in corneal ulcer and ocular surface disorder patients. A total of 35 patients of corneal ulcer, 20 patients of ocular surface disorders and 10 control subjects were included in this study and estimation of active form of MMP-2 and MMP-9 was done by gelatin zymography. Tear samples were collected by capillary tube method. Both pro- and active forms of MMP-9 were detected in 24 out of 35 patients with corneal ulcer and 15 out of 20 patients with ocular surface disorders. None of the patients were showing MMP-2 activity. Neither MMP-2 nor MMP-9 was detected in the control group. Active forms of MMP-9 are present in tears of severe ulcerative and ocular surface disorder patients. Thus, proteinase inhibitors have been recommended for the treatment of corneal ulcer and ocular surface disorders to reduced the progression of stromal ulcer and to minimize corneal scarring.
本文的目的是确定角膜溃疡和眼表疾病患者中基质金属蛋白酶（MMP - 2和MMP - 9）的活性形式。本研究共纳入35例角膜溃疡患者、20例眼表疾病患者和10例对照受试者，并通过明胶酶谱法对MMP - 2和MMP - 9的活性形式进行评估。采用毛细管法收集泪液样本。在35例角膜溃疡患者中的24例以及20例眼表疾病患者中的15例中检测到了MMP - 9的前体形式和活性形式。所有患者均未表现出MMP - 2活性。在对照组中未检测到MMP - 2和MMP - 9。严重溃疡性和眼表疾病患者的泪液中存在MMP - 9的活性形式。因此，推荐使用蛋白酶抑制剂治疗角膜溃疡和眼表疾病，以减少基质溃疡的进展并使角膜瘢痕最小化。
