Musser Jeffrey M B, Taylor Charles A, Guo Jianhua, Tizard Ian R, Walker John W
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843-4467, USA.
Am J Vet Res. 2008 Oct;69(10):1366-70. doi: 10.2460/ajvr.69.10.1366.
To identify a strain of contagious ecthyma virus from goats that possesses the appropriate characteristics for an effective vaccine for goats.
25 goat kids used for vaccine development and 100 goat kids used for evaluation of vaccine efficacy.
5 strains of contagious ecthyma virus were tested in a vaccination-challenge study to identify the best strain to be the seed strain for a contagious ecthyma vaccine. The vaccine derived from the chosen viral stain was tested at 2 concentrations for efficacy in a vaccination-challenge study.
2 of 5 viral strains induced moderate to severe scabs following infection, and 3 viral strains protected the goats from wild-type virus challenge following vaccination. Viral strain 47CE was selected as the seed source for the production of a contagious ecthyma vaccine because of the larger vaccine-to-challenge scab formation ratio. Vaccine 47CE protected all goat kids (48/48) following challenge with the wild-type contagious ecthyma virus; all goat kids (32/32) in the control group had scab formation following challenge with the wild-type contagious ecthyma virus, which indicated no protection following administration of vaccine diluent.
A vaccine containing a caprine strain of contagious ecthyma virus used in goats appeared to provide the characteristics needed for an effective vaccine, including good scab production and protection from wild-type infection. This vaccine may potentially provide better protection for goats from contagious ecthyma than currently available vaccines labeled for sheep.
从山羊中鉴定出一株具有适合作为有效山羊疫苗特性的传染性脓疱病毒。
25只用于疫苗研发的山羊幼崽和100只用于评估疫苗效力的山羊幼崽。
在一项接种-攻毒研究中对5株传染性脓疱病毒进行测试，以确定最适合作为传染性脓疱疫苗种子毒株的毒株。在接种-攻毒研究中，对源自所选病毒毒株的疫苗进行了两种浓度的效力测试。
5株病毒中有2株在感染后引起中度至重度结痂，3株病毒在接种疫苗后保护山羊免受野生型病毒攻击。由于疫苗与攻毒后结痂形成比例更大，47CE病毒株被选为生产传染性脓疱疫苗的种子来源。用野生型传染性脓疱病毒攻毒后，47CE疫苗保护了所有山羊幼崽（48/48）；对照组所有山羊幼崽（32/32）在接受野生型传染性脓疱病毒攻毒后都出现了结痂，这表明接种疫苗稀释液后没有保护作用。
一种用于山羊的含有山羊传染性脓疱病毒株的疫苗似乎具备有效疫苗所需的特性，包括良好的结痂产生和对野生型感染的保护。这种疫苗可能比目前用于绵羊的现有疫苗能为山羊提供更好的传染性脓疱防护。
