Dutu R, Maltezeanu G
Acta Histochem. 1976;57(1):34-43. doi: 10.1016/S0065-1281(76)80005-0.
Wistar rats were intraperitoneally treated with different doses of Aflatoxin B1 (5 and 7 mg/kg body weight) and sacrificed after various periods (6, 24, 48, 72 h, 5, 6, 7, 8, 9, 10 days and 86, 96, 97 weeks) in an attempt to detect liver, lung, intestine, spleen, mesenteric lymph nodes alterations. There are histochemical indications to suggest early liver changes for both doses in carbohydrates metabolism (decreasing of: polysaccharides, acid and neutral mucopolysaccharides, LD activity diffusely distributed through the all lobule). After 86, 96, 97 weeks from Aflatoxin administration, hepatocytes alterations rendered evident by fat accumulations, depletion or loss of polysaccharides, acid and neutral mucopolysaccharides, loss of cytoplasmic enzymes activities, altered pattern of surface ATP-ase are associated with diffuse fibrosis. The findings are suggestive that liver alterations are depending on dose and duration of exposure and could be detected by histochemical methods before significant morphological changes appear.
将不同剂量的黄曲霉毒素B1（5和7毫克/千克体重）腹腔注射给Wistar大鼠，并在不同时间段（6、24、48、72小时，5、6、7、8、9、10天以及86、96、97周）后处死，以检测肝脏、肺、肠道、脾脏、肠系膜淋巴结的变化。有组织化学迹象表明，两种剂量在碳水化合物代谢方面均提示早期肝脏变化（多糖、酸性和中性粘多糖减少，LD活性在整个小叶中弥漫分布）。在给予黄曲霉毒素86、96、97周后，肝细胞的变化表现为脂肪堆积、多糖、酸性和中性粘多糖的消耗或丧失、细胞质酶活性丧失、表面ATP酶模式改变，并伴有弥漫性纤维化。这些发现表明，肝脏变化取决于剂量和暴露持续时间，并且在明显的形态学变化出现之前，可以通过组织化学方法检测到。
