Wang Jing, Meng Wenying, Zheng Xiaofeng, Liu Shanli, Li Genxi
Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China.
Biosens Bioelectron. 2009 Feb 15;24(6):1598-602. doi: 10.1016/j.bios.2008.08.030. Epub 2008 Aug 27.
In this paper, we report a novel electrochemical detection approach for platelet-derived growth factor (PDGF) via "sandwich" structure and gold nanoparticles (Au-NPs) mediated amplification technique. The "sandwich" structure is fabricated based on the fact that PDGF has two aptamer-binding sites, which makes it possible for one PDGF molecule to connect with two aptamers simultaneously. It is found that this electrochemical system with "sandwich" structure and Au-NPs can significantly amplify the signal of electrochemical probe Ru(NH(3))(5)Cl for PDGF detection, and thus increase the detection sensitivity significantly. As a result, this PDGF detection approach obtains an extraordinarily low detection limit of 1 x 10(-14)M for purified samples, 1 x 10(-12)M for contaminated-ridden samples or undiluted blood serum. This detection approach can also exhibit good stability and excellent specificity.
在本文中，我们报道了一种通过“三明治”结构和金纳米颗粒（Au-NPs）介导的放大技术对血小板衍生生长因子（PDGF）进行新型电化学检测的方法。“三明治”结构基于PDGF具有两个适配体结合位点这一事实构建而成，这使得一个PDGF分子能够同时与两个适配体相连。研究发现，这种具有“三明治”结构和Au-NPs的电化学系统能够显著放大用于检测PDGF的电化学探针Ru(NH(3))(5)Cl的信号，从而显著提高检测灵敏度。结果，这种PDGF检测方法对于纯化样品获得了极低的检测限，为1×10(-14)M，对于污染严重的样品或未稀释的血清为1×10(-12)M。这种检测方法还具有良好的稳定性和出色的特异性。
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