Suprun Elena, Shumyantseva Victoria, Bulko Tatiana, Rachmetova Svetlana, Rad'ko Sergei, Bodoev Nikolay, Archakov Alexander
Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya Street 10, Moscow 119121, Russia.
Biosens Bioelectron. 2008 Dec 1;24(4):831-6. doi: 10.1016/j.bios.2008.07.008. Epub 2008 Jul 15.
A novel electrochemical method for the detection of bioaffinity interactions based on a gold-nanoparticles sensing platform and on the usage of stripping voltammetry technique was developed. The oxidation of gold surface (resulted in gold oxide formation) upon polarization served as a basis for analytical response. As a model, thrombin-thrombin binding aptamer couple was chosen. The aptamer was immobilized on a screen-printed electrode modified with gold-nanoparticles by avidin-biotin technology. Cathodic peak area was found proportional to thrombin quantity specifically adsorbed onto electrode surface. Sigmoid calibration curve as is typical for immunoassay was obtained, with thrombin detection limit of 10(-9)M. Linear range corresponds from 10(-8) to 10(-5)M thrombin concentration or 2 x 10(-14) to 2 x 10(-11)mol/electrode (R=0.996). Binding of thrombin to an aptamer has also been detected using the ferricyanide/ferrocyanide redox couple as electrochemical indicator.
开发了一种基于金纳米颗粒传感平台和溶出伏安法技术检测生物亲和相互作用的新型电化学方法。极化时金表面的氧化（导致形成金氧化物）作为分析响应的基础。作为模型，选择了凝血酶-凝血酶结合适体对。通过抗生物素蛋白-生物素技术将适体固定在用金纳米颗粒修饰的丝网印刷电极上。发现阴极峰面积与特异性吸附在电极表面的凝血酶量成正比。获得了免疫测定中典型的S形校准曲线，凝血酶检测限为10(-9)M。线性范围对应于凝血酶浓度10(-8)至10(-5)M或2×10(-14)至2×10(-11)mol/电极（R = 0.996）。还使用铁氰化物/亚铁氰化物氧化还原对作为电化学指示剂检测了凝血酶与适体的结合。
