Li Bingling, Wang Yuling, Wei Hui, Dong Shaojun
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, PR China.
Biosens Bioelectron. 2008 Feb 28;23(7):965-70. doi: 10.1016/j.bios.2007.09.019. Epub 2007 Oct 2.
Here, we report a sensitive amplified electrochemical impedimetric aptasensor for thrombin, a kind of serine protease that plays important role in thrombosis and haemostasis. For improving detection sensitivity, a sandwich sensing platform is fabricated, in which the thiolated aptamers are firstly immobilized on a gold substrate to capture the thrombin molecules, and then the aptamer functionalized Au nanoparticles (AuNPs) are used to amplify the impedimetric signals. Such designed aptamer/thrombin/AuNPs sensing system could not only improve the detection sensitivity compared to the reported impedimetric aptasensors but also provide a promising signal amplified model for aptamer-based protein detection. In this paper, we realize a sensitive detection limit of 0.02 nM, with a linear range of 0.05-18 nM. Meanwhile, the effect of 6-mercaptohexanol (MCH) and 2-mercaptoethanol (MCE) on the modification of the electrode is investigated.
在此，我们报道了一种用于凝血酶的灵敏的电化学阻抗适配体传感器，凝血酶是一种在血栓形成和止血过程中起重要作用的丝氨酸蛋白酶。为提高检测灵敏度，构建了一种夹心传感平台，其中首先将硫醇化适配体固定在金基底上以捕获凝血酶分子，然后使用适配体功能化的金纳米颗粒（AuNPs）来放大阻抗信号。这种设计的适配体/凝血酶/AuNPs传感系统不仅与已报道的阻抗适配体传感器相比提高了检测灵敏度，而且为基于适配体的蛋白质检测提供了一种有前景的信号放大模型。在本文中，我们实现了0.02 nM的灵敏检测限，线性范围为0.05 - 18 nM。同时，研究了6 - 巯基己醇（MCH）和2 - 巯基乙醇（MCE）对电极修饰的影响。
