裸露及包裹DNA的碳纳米管在自组装单分子层表面的吸附与释放行为

Adsorption and release behavior of bare and DNA-wrapped-carbon nanotubes on self-assembled monolayer surface.

作者信息

Zheng Dan, Li Xueling, Ye Jianshan

机构信息

College of Chemistry and Chemical Technology, South China University of Technology, Guangzhou 510640, PR China.

出版信息

Bioelectrochemistry. 2009 Feb;74(2):240-5. doi: 10.1016/j.bioelechem.2008.08.006. Epub 2008 Aug 28.

DOI:10.1016/j.bioelechem.2008.08.006

PMID:18829397

Abstract

The adsorption and release behavior of single-stranded DNA-wrapped single-walled carbon nanotubes (ssDNA-w-SWCNTs) on alkylthiol self-assembled monolayer (SAM) surface was systematically characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Fast electron transfer between bare Au electrode and redox species blocked by the alkylthiol SAM can be restored by SWCNTs or ssDNA-w-SWCNTs. The release of ssDNA-w-SWCNTs is carried out by positive or negative desorption potential. SWCNTs/SAM or ssDNA-w-SWCNTs/SAM is completely removed from Au surface at +0.90 V or -1.40 V (vs. 3.0 M KCl|Ag|AgCl). The controlled release of SWCNTs/SAM and ssDNA-w-SWCNTs/SAM holds great promise for gene delivering.

摘要

通过循环伏安法（CV）和电化学阻抗谱（EIS）系统地表征了单链DNA包裹的单壁碳纳米管（ssDNA-w-SWCNTs）在烷基硫醇自组装单分子层（SAM）表面的吸附和释放行为。被烷基硫醇SAM阻断的裸金电极与氧化还原物种之间的快速电子转移可通过单壁碳纳米管或单链DNA包裹的单壁碳纳米管恢复。单链DNA包裹的单壁碳纳米管的释放通过正或负解吸电位进行。在+0.90 V或-1.40 V（相对于3.0 M KCl|Ag|AgCl）时，单壁碳纳米管/自组装单分子层或单链DNA包裹的单壁碳纳米管/自组装单分子层从金表面完全去除。单壁碳纳米管/自组装单分子层和单链DNA包裹的单壁碳纳米管/自组装单分子层的可控释放对基因递送具有很大的前景。