Bryant D K, Orlando R C, Fenselau C, Sowder R C, Henderson L E
Department of Chemistry and Biochemistry, University of Maryland Baltimore County 21228.
Anal Chem. 1991 Jun 1;63(11):1110-4. doi: 10.1021/ac00011a010.
A number of phosphatidylcholines have been isolated from an HIV-1/MN preparation by reversed-phase high-performance liquid chromatography (HPLC) and analyzed by fast atom bombardment mass spectrometry (FABMS), FABMS/MS, and FABMS/MS/MS in both positive- and negative-ion modes. Negative-ion FABMS/MS with high-energy collisions was used to identify the length of the acyl groups and the degree of saturation, as well as their position on the glyceride group. FABMS/MS in the positive-ion mode was used to identify the polar head group. Negative-ion FABMS/MS/MS was used to locate positions of double bonds in acyl groups. We find that four-sector tandem mass spectrometry with high-energy collisional activation provides qualitative analysis of viral phosphatidyl lipids in considerable detail, as well as semiquantitative information. Approximate quantitation of the phosphatidylcholine content of the HIV-1/MN preparation by measuring relative peak heights of molecular ions in FABMS reveals an array of phosphatidylcholines consistent with that found in human erythrocytes, indicating the likely source of lipids in the viral membrane to be the host cell membrane.
通过反相高效液相色谱法（HPLC）从HIV-1/MN制剂中分离出多种磷脂酰胆碱，并采用快原子轰击质谱法（FABMS）、串联快原子轰击质谱法（FABMS/MS）和串联二级快原子轰击质谱法（FABMS/MS/MS）在正离子和负离子模式下进行分析。采用具有高能碰撞的负离子FABMS/MS来鉴定酰基的长度、饱和度以及它们在甘油酯基团上的位置。采用正离子模式的FABMS/MS来鉴定极性头部基团。采用负离子FABMS/MS/MS来确定酰基中双键的位置。我们发现，具有高能碰撞活化的四扇区串联质谱法能够相当详细地对病毒磷脂进行定性分析，并提供半定量信息。通过测量FABMS中分子离子的相对峰高对HIV-1/MN制剂中的磷脂酰胆碱含量进行近似定量，结果显示出一系列与人类红细胞中发现的磷脂酰胆碱一致的磷脂酰胆碱，这表明病毒膜中脂质的可能来源是宿主细胞膜。
