Ramaneswari K, Rao L M
Department of Zoology, Andhra University, Visakhapatnam, India.
J Environ Biol. 2008 Mar;29(2):183-5.
The response of NADPH cytochrome C reductase (NCCR) activity in liver of Labeo rohita fish exposed to the pesticides, 0.25 microgl(-1) endosulfan and 2 mg/l monocrotophos was studied. In terms of specific enzyme activity (mU/mg protein) a significant level of NCCR was observed in the liver tissues of Labeo rohita exposed to the pesticides, when compared to the control fish (2.460 mU/mg protein). Increase of NCCR activity was more in the liver of the fish exposed to monocrotophos (4.595 mU/mg protein) than those exposed to endosulfan (2.850 mU/mg protein). The results demonstrate that the pesticides, endosulfan and monocrotophos, interfere with NADPH dependent monoxygenase mechanism and are effective inducers of NADPH cytochrome C reductase. The activity of NCCR in the liver tissue of Labeo rohita may serve as a useful tool for monitoring aquatic pollution.
研究了暴露于农药（0.25微克/升硫丹和2毫克/升久效磷）的露斯塔野鲮肝脏中NADPH细胞色素C还原酶（NCCR）的活性。就比酶活性（毫微单位/毫克蛋白质）而言，与对照鱼（2.460毫微单位/毫克蛋白质）相比，在暴露于农药的露斯塔野鲮肝脏组织中观察到显著水平的NCCR。暴露于久效磷的鱼肝脏中NCCR活性的增加（4.595毫微单位/毫克蛋白质）比暴露于硫丹的鱼（2.850毫微单位/毫克蛋白质）更多。结果表明，农药硫丹和久效磷干扰NADPH依赖性单加氧酶机制，是NADPH细胞色素C还原酶的有效诱导剂。露斯塔野鲮肝脏组织中NCCR的活性可作为监测水体污染的有用工具。
