Blanco M, Pérez-Martín R I, Sotelo C G
Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, Eduardo Cabello 6, 36208 Vigo, Spain.
J Agric Food Chem. 2008 Nov 12;56(21):9868-74. doi: 10.1021/jf8015128. Epub 2008 Oct 3.
The identification of commercial shark species is a relevant issue to ensure the correct labeling of seafood products, to maintain consumer confidence in seafood, and to enhance the knowledge of the species and volumes that are at present being captured, thus improving the management of shark fisheries. The polymerase chain reaction was employed to obtain a 423 bp amplicon from the mitochondrial cytochrome b gene. The sequences from this fragment, belonging to 63 authentic individuals of 23 species, were analyzed using a genetic distance method. Nine different samples of commercial fresh, frozen, and convenience food were obtained in local and international markets to validate the methodology. These samples were analyzed, and sequences were employed for species identification, showing that forensically informative nucleotide sequencing (FINS) is a suitable technique for identification of processed seafood containing shark as an ingredient. The results also showed that incorrect labeling practices may occur regarding shark products, probably because of incorrect labeling at the production point.
识别商业鲨鱼种类是一个相关问题，对于确保海鲜产品的正确标签、维持消费者对海鲜的信心、增进对目前被捕捞的鲨鱼种类和数量的了解从而改善鲨鱼渔业管理而言至关重要。采用聚合酶链反应从线粒体细胞色素b基因中获得一个423 bp的扩增子。使用遗传距离方法分析了来自23个物种的63个真实个体的该片段序列。在本地和国际市场获取了9种不同的商业新鲜、冷冻和方便食品样本以验证该方法。对这些样本进行了分析，并将序列用于物种鉴定，结果表明法医信息核苷酸测序（FINS）是鉴定含有鲨鱼成分的加工海鲜的合适技术。结果还表明鲨鱼产品可能存在标签错误行为，这可能是由于生产环节标签错误所致。
