Pirooznia Mehdi, Gong Ping, Yang Jack Y, Yang Mary Qu, Perkins Edward J, Deng Youping
Department of Biological Sciences, University of Southern Mississippi, Hattiesburg, MS 39406, USA.
BMC Genomics. 2008 Sep 16;9 Suppl 2(Suppl 2):S11. doi: 10.1186/1471-2164-9-S2-S11.
Microarray technology is widely applied to address complex scientific questions. However, there remain fundamental issues on how to design experiments to ensure that the resulting data enables robust statistical analysis. Interwoven loop design has several advantages over other designs. However it suffers in the complexity of design. We have implemented an online web application which allows users to find optimal loop designs for two-color microarray experiments. Given a number of conditions (such as treatments or time points) and replicates, the application will find the best possible design of the experiment and output experimental parameters. It is freely available from http://mcbc.usm.edu/iloop.
微阵列技术被广泛应用于解决复杂的科学问题。然而，在如何设计实验以确保所得数据能够进行可靠的统计分析方面，仍然存在一些基本问题。与其他设计相比，交织环设计具有若干优势。然而，它在设计复杂性方面存在不足。我们已经实现了一个在线网络应用程序，该程序允许用户为双色微阵列实验找到最优的环设计。给定一些条件（如处理或时间点）和重复次数，该应用程序将找到最佳的实验设计并输出实验参数。可从http://mcbc.usm.edu/iloop免费获取该程序。
