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从牛奶样本中分离出的乳房链球菌和副乳房链球菌的鉴定及抗菌药物耐药性

Identification and antimicrobial resistance of Streptococcus uberis and Streptococcus parauberis isolated from bovine milk samples.

作者信息

Pitkälä A, Koort J, Björkroth J

机构信息

Finnish Food Safety Authority Evira, Helsinki, Finland.

出版信息

J Dairy Sci. 2008 Oct;91(10):4075-81. doi: 10.3168/jds.2008-1040.

DOI:10.3168/jds.2008-1040
PMID:18832235
Abstract

The conventional identification of Streptococcus uberis/parauberis group (n = 137) in clinical and subclinical bovine mastitis samples originating from 111 different farms was compared with identification based on 16 and 23S rRNA gene HindIII RFLP patterns used as operational taxonomic units in numerical analyses. On the basis of ribopattern analysis only 2 isolates belonged to S. parauberis, which is thus not a frequent cause of bovine intramammary infections in Finland. According to in vitro antimicrobial susceptibility testing, Streptococcus uberis is susceptible to beta-lactam antibiotics. The prevalence of erythromycin (15.6%) and oxytetracycline (40.6%) resistance of clinical S. uberis isolates was higher than reported previously among subclinical isolates. The 2 subclinical S. parauberis isolates were susceptible to all the antimicrobials tested.

摘要

将源自111个不同农场的临床和亚临床型牛乳腺炎样本中乳房链球菌/副乳房链球菌组(n = 137)的传统鉴定方法,与基于16S和23S rRNA基因HindIII限制性片段长度多态性(RFLP)模式的鉴定方法进行了比较,后者在数值分析中用作操作分类单位。基于核糖体模式分析,只有2株分离株属于副乳房链球菌,因此在芬兰它并非牛乳房内感染的常见病因。根据体外抗菌药敏试验,乳房链球菌对β-内酰胺类抗生素敏感。临床乳房链球菌分离株中红霉素(15.6%)和土霉素(40.6%)的耐药率高于先前报道的亚临床分离株。2株亚临床副乳房链球菌分离株对所有测试抗菌药物均敏感。

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