Park Yon-Kyoung, Nho Seong-Won, Shin Gee-Wook, Park Seong-Bin, Jang Ho-Bin, Cha In-Seok, Ha Mi-Ae, Kim Young-Rim, Dalvi Rishikesh S, Kang Bong-Jo, Jung Tae-Sung
Research Institute of Life Science, College of Veterinary medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Vet Microbiol. 2009 Apr 14;136(1-2):76-81. doi: 10.1016/j.vetmic.2008.10.002. Epub 2008 Oct 14.
The rates of antibiotic susceptibility and resistance were investigated in Streptococcus iniae and Streptococcus parauberis isolates obtained from diseased olive flounders (Paralichthys olivaceus) collected from fish farms in Jeju Island, Korea. Isolates of S. iniae (n=65) were susceptible to cefotaxime, erythromycin, ofloxacin, penicillin, tetracycline and vancomycin, as demonstrated by the minimum inhibitory concentration (MIC) test. Isolates of S. parauberis (n=86) were highly resistant to erythromycin (58% of the 86 isolates tested) and tetracycline (63% of the 86 isolates tested). Fifty-four isolates of tetracycline-resistant S. parauberis contained the tet(M/O/S) genes, of which 39 and 12 isolates contained the tet(M) and tet(S) genes, respectively, whereas 3 isolates contained both the tet(M) and tet(S) genes. Among the erythromycin-resistant isolates of S. parauberis (n=50) only 14 contained the erm(B) gene. These results suggest that the tet(S) and erm(B) genes of S. parauberis are involved in the acquisition of high-level resistance to erythromycin and tetracycline. Our findings reveal a high rate of antibiotic resistance among strains of S. parauberis and emphasize the need to develop an appropriate vaccine to reduce the use of antibiotics.
对从韩国济州岛养鱼场采集的患病牙鲆（Paralichthys olivaceus）中分离出的海豚链球菌和副乳房链球菌菌株的抗生素敏感性和耐药率进行了调查。通过最低抑菌浓度（MIC）试验表明，海豚链球菌分离株（n = 65）对头孢噻肟、红霉素、氧氟沙星、青霉素、四环素和万古霉素敏感。副乳房链球菌分离株（n = 86）对红霉素（86株受试菌株中的58%）和四环素（86株受试菌株中的63%）高度耐药。54株四环素耐药副乳房链球菌分离株含有tet（M/O/S）基因，其中39株和12株分别含有tet（M）和tet（S）基因，而3株同时含有tet（M）和tet（S）基因。在副乳房链球菌的红霉素耐药分离株（n = 50）中，只有14株含有erm（B）基因。这些结果表明，副乳房链球菌的tet（S）和erm（B）基因与获得对红霉素和四环素的高水平耐药性有关。我们的研究结果揭示了副乳房链球菌菌株中抗生素耐药率很高，并强调需要开发合适的疫苗以减少抗生素的使用。
