Lin Z, Laneuville O, Pace-Asciak C R
Research Institute, Hospital for Sick Children, Toronto, Canada.
Biochem Biophys Res Commun. 1991 Aug 30;179(1):52-6. doi: 10.1016/0006-291x(91)91332-7.
In this paper we show that hepoxilin A3 induces the expression of heat shock protein expression in human neutrophils at a concentration of 100 nM using Western blotting techniques employing the use of a commercial monoclonal antibody to HSP72. No regiospecificity was observed as the 8S enantiomer of HxA3 was as active as the 8R enantiomer of HxA3. Comparison of the effects of HxA3 with 12S-HETE and PGA1 indicated that HxA3 was as effective as 12S-HETE although PGA1 was essentially inactive at the same concentration used for these 12-lipoxygenase products.
在本文中，我们使用针对HSP72的商业单克隆抗体，通过蛋白质免疫印迹技术表明，在浓度为100 nM时，肝氧素A3可诱导人中性粒细胞中热休克蛋白的表达。未观察到区域特异性，因为HxA3的8S对映体与HxA3的8R对映体活性相同。将HxA3与12S-HETE和PGA1的作用进行比较表明，HxA3与12S-HETE一样有效，尽管PGA1在用于这些12-脂氧合酶产物的相同浓度下基本无活性。
