Rifkin B R, Vernillo A T, Kleckner A P, Auszmann J M, Rosenberg L R, Zimmerman M
Department of Oral Medicine and Pathology, New York University College of Dentistry, New York 10010.
Biochem Biophys Res Commun. 1991 Aug 30;179(1):63-9. doi: 10.1016/0006-291x(91)91334-9.
Cathepsin B and L activities were examined with chicken osteoclasts isolated by sequential filtration and inhibitors were added to disaggregated rat osteoclasts on cortical bovine bone. Z-Phe-Phe-CHN2, a selective inhibitor of cathepsin L, at 1, 5, and 10 microM, inhibited bone resorption by rat osteoclasts 50, 85, and 100 per cent and, in chicken osteoclasts, cathepsin L activity was comparably inhibited. Cathepsin L in avian osteoclasts was also 25-fold higher than cathepsin B. Chicken osteoclasts treated with Z-Phe-Ala-CHN2, a generalized cysteine proteinase inhibitor, had both cathepsins inhibited to the same extent. Cathepsin L may play a key role in resorption.
通过连续过滤分离鸡破骨细胞，检测组织蛋白酶B和L的活性，并将抑制剂添加到皮质牛骨上分散的大鼠破骨细胞中。Z-Phe-Phe-CHN2是一种组织蛋白酶L的选择性抑制剂，浓度为1、5和10微摩尔时，分别抑制大鼠破骨细胞骨吸收50%、85%和100%，在鸡破骨细胞中，组织蛋白酶L的活性也受到类似抑制。禽类破骨细胞中的组织蛋白酶L也比组织蛋白酶B高25倍。用Z-Phe-Ala-CHN2（一种通用的半胱氨酸蛋白酶抑制剂）处理鸡破骨细胞，两种组织蛋白酶受到同等程度的抑制。组织蛋白酶L可能在骨吸收中起关键作用。
