Jahan-Parwar Babak, Chhetri Dinesh K, Ye Ming, Hart Steven, Berke Gerald S
Division of Head and Neck Surgery, University of California-Los Angeles Medical Center, Los Angeles, California 90095, USA.
Ann Otol Rhinol Laryngol. 2008 Sep;117(9):703-7. doi: 10.1177/000348940811700913.
We evaluated cross-linked hyaluronic acid (hylan B gel) as a scaffold for tissue regeneration and mucosal wave restoration in carbon dioxide laser-ablated canine vocal folds.
Five beagles underwent stroboscopy before ablation of the left vocal fold with a carbon dioxide laser. Four weeks later, stroboscopy was repeated before and after submucosal injection of hylan B gel into the left vocal fold of 4 animals and of saline solution in 1 animal. Stroboscopy was repeated 12 weeks later, and histologic analysis was performed.
Four weeks after laser ablation, all animals had soft tissue defects and absence of mucosal waves. Hylan B injection restored mucosal waves, and saline injection did not. Twelve weeks after injection, hylan B-injected larynges had tissue regeneration and mucosal waves, and the saline-injected larynx had neither. Histology showed regenerated lamina propria with residual foci of hylan B in the hylan B-injected larynges and dense submucosal scar in the saline-injected animal.
Submucosal hylan B gel injection in laser-ablated canine vocal folds restored tissue volume and mucosal waves and facilitated functional tissue regeneration over 12 weeks. Hylan B gel may have utility as a soft tissue scaffold for rehabilitation of phonatory function in vocal folds with lamina propria defects.
我们评估了交联透明质酸（hylan B凝胶）作为二氧化碳激光消融犬声带组织再生和黏膜波恢复支架的效果。
五只比格犬在使用二氧化碳激光消融左侧声带前接受频闪喉镜检查。四周后，对4只动物的左侧声带黏膜下注射hylan B凝胶，对1只动物的左侧声带黏膜下注射生理盐水，在注射前后再次进行频闪喉镜检查。12周后再次进行频闪喉镜检查，并进行组织学分析。
激光消融四周后，所有动物均出现软组织缺损且无黏膜波。注射hylan B凝胶恢复了黏膜波，而注射生理盐水则未恢复。注射12周后，注射hylan B凝胶的喉部出现组织再生和黏膜波，而注射生理盐水的喉部两者均未出现。组织学显示，注射hylan B凝胶的喉部有再生的固有层，其中残留有hylan B病灶，而注射生理盐水的动物则有致密的黏膜下瘢痕。
在激光消融的犬声带中黏膜下注射hylan B凝胶可恢复组织体积和黏膜波，并在12周内促进功能性组织再生。Hylan B凝胶可能作为一种软组织支架用于固有层缺损声带发声功能的康复。
