Magnotti Ralph A, Connell Jodi L, Marietta Peter M
IDEXX Laboratories, Inc., One IDEXX Drive, Westbrook, ME 04092, USA.
Clin Chim Acta. 2009 Jan;399(1-2):59-63. doi: 10.1016/j.cca.2008.09.009. Epub 2008 Sep 14.
NSF (nephrogenic systemic fibrosis) is a potentially serious adverse effect for renal patients undergoing MRI (magnetic resonance imaging) procedures using gadolinium-containing contrast agents. There is therefore a need to verify clearance of these agents and to confirm appropriate renal status of patients treated with these drugs.
Serum samples from canine and feline subjects dosed with 0.1 mmol/kg of gadolinium agent, or from spiked samples were assayed for gadopentetate (Magnevist), gadodiamide (Omniscan) or gadoversetamide (OptiMARK) using a new dye reagent on the Olympus AU400. Accuracy was verified by ICP-MS.
The reportable dynamic range is 3-600 micromol/l Gd. Split serum samples from animals dosed with 0.1 mmol/kg of gadopentetate ranged from 7-458 micromol/l Gd: y=1.121x+0.267, r=0.996, for the Olympus method as a function of Gd measurement by ICP-MS. Between-day imprecision was 1.3% CV-3.6% CV for samples ranging from 12-400 micromol/l Gd.
The assay is useful to verify the clearance of gadolinium and for evaluation of renal status by estimation of GFR using gadopentetate.
肾源性系统性纤维化（NSF）是接受使用含钆造影剂的磁共振成像（MRI）检查的肾病患者可能出现的严重不良反应。因此，有必要验证这些造影剂的清除情况，并确认接受这些药物治疗的患者的肾脏状况是否合适。
对给予0.1 mmol/kg钆造影剂的犬类和猫类动物的血清样本，或加标样本，使用奥林巴斯AU400上的一种新型染料试剂，检测钆喷酸葡胺（马根维显）、钆双胺（欧乃影）或钆贝葡胺（优化磁共振）。通过电感耦合等离子体质谱法（ICP-MS）验证准确性。
可报告的动态范围为3 - 600 μmol/l钆。给予0.1 mmol/kg钆喷酸葡胺的动物的分样血清样本中钆含量范围为7 - 458 μmol/l钆：对于奥林巴斯方法，y = 1.121x + 0.267，r = 0.996，作为通过ICP-MS测量钆的函数。对于12 - 400 μmol/l钆的样本，日间不精密度为1.3%CV - 3.6%CV。
该检测方法有助于验证钆的清除情况，并通过使用钆喷酸葡胺估计肾小球滤过率来评估肾脏状况。
