Blanco Helga M, Lacoste María G, Eliçabe Ricardo J, Di Genaro María S
Immunology, Laboratory of Microbiology, Chemistry, Biochemistry and Pharmacy Faculty, National University of San Luis, IMIBIO-SL, CONICET, San Luis, Argentina.
Vaccine. 2008 Dec 2;26(51):6497-502. doi: 10.1016/j.vaccine.2008.09.046.
Yersinia enterocolitica (Ye) mutant strain (sycH-) is unable to secrete the virulence protein YopH. Mucosal vaccination is often required to induce protection, but stimulating strong IgA response is frequently difficult. Here, we addressed whether Ye sycH- might induce IgA response, and investigated its attenuation in TNFRp55-/-, IL-12p40-/- and IL-4-/- mice. We found that Ye sycH- colonizes Peyer's patches, and induces higher Yersinia-specific IgA levels in feces and in serum compared with Ye wild type. The Ye sycH-mutant proved to be attenuated and induced IgA in both wild-type and immunodeficient mice. These lines of evidence show the attenuation of Ye sycH- and its ability to stimulate an IgA response. This mutant might be useful as an oral vaccine carrier.
小肠结肠炎耶尔森菌（Ye）突变株（sycH-）无法分泌毒力蛋白YopH。通常需要进行黏膜疫苗接种来诱导保护作用，但刺激产生强烈的IgA反应常常很困难。在此，我们探讨了Ye sycH-是否能诱导IgA反应，并研究了其在TNFRp55-/-、IL-12p40-/-和IL-4-/-小鼠中的减毒情况。我们发现Ye sycH-定殖于派尔集合淋巴结，与Ye野生型相比，其在粪便和血清中诱导产生更高水平的耶尔森菌特异性IgA。Ye sycH-突变株被证明具有减毒作用，并能在野生型和免疫缺陷小鼠中诱导产生IgA。这些证据表明Ye sycH-的减毒作用及其刺激IgA反应的能力。该突变株可能作为口服疫苗载体有用。
