共生细菌分泌促胰岛素蛋白：重塑肠道以治疗糖尿病。
Secretion of insulinotropic proteins by commensal bacteria: rewiring the gut to treat diabetes.
作者信息
Duan Faping, Curtis Katherine L, March John C
机构信息
Department of Biological and Environmental Engineering, Cornell University, Ithaca, New York 14853, USA.
出版信息
Appl Environ Microbiol. 2008 Dec;74(23):7437-8. doi: 10.1128/AEM.01019-08. Epub 2008 Oct 3.
Abstract
Here, we show that commensal bacteria can stimulate intestinal epithelial cells to secrete insulin in response to glucose. Commensal strains were engineered to secrete the insulinotropic proteins GLP-1 and PDX-1. Epithelia stimulated by engineered strains and glucose secreted up to 1 ng ml(-1) of insulin with no significant background secretion.
摘要
在此，我们表明共生细菌可刺激肠道上皮细胞响应葡萄糖分泌胰岛素。对共生菌株进行改造使其分泌促胰岛素蛋白胰高血糖素样肽-1（GLP-1）和胰腺十二指肠同源盒-1（PDX-1）。经改造后的菌株和葡萄糖刺激的上皮细胞分泌的胰岛素高达1纳克/毫升，且无明显的背景分泌。
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