Cronenberg C C, van den Heuvel J C
University of Amsterdam, Department of Chemical Engineering & Biotechnological Centre, The Netherlands.
Biosens Bioelectron. 1991;6(3):255-62. doi: 10.1016/0956-5663(91)80011-l.
A glucose micro-electrode was developed for direct measurements inside biofilms, and applied for the determination of effective diffusion coefficients in a model system of agar beads containing immobilized yeast cells. Two methods were used, one based on concentration gradients present at the liquid/solid interface of an active biofilm under steady-state conditions, the other based on the rate of glucose redistribution in an inactivated biofilm under transient-state conditions. Additional measurements with pH and oxygen micro-electrodes were performed and thus allowed for in-situ correction of the glucose electrode signal. From the micro-electrode measurements in the model system it was concluded that the glucose micro-sensor is a useful tool with which to obtain effective diffusion coefficients in biofilms.
开发了一种用于在生物膜内部进行直接测量的葡萄糖微电极，并将其应用于测定含有固定化酵母细胞的琼脂珠模型系统中的有效扩散系数。使用了两种方法，一种基于稳态条件下活性生物膜液/固界面处存在的浓度梯度，另一种基于瞬态条件下失活生物膜中葡萄糖重新分布的速率。使用pH和氧微电极进行了额外的测量，从而可以对葡萄糖电极信号进行原位校正。从模型系统中的微电极测量结果得出结论，葡萄糖微传感器是一种用于获取生物膜中有效扩散系数的有用工具。
