Kristensen Jan B, Felby Claus, Jørgensen Henning
Forest and Landscape Denmark, University of Copenhagen, Rolighedsvej 23, DK-1958 Frederiksberg, Denmark.
Appl Biochem Biotechnol. 2009 May;156(1-3):127-32. doi: 10.1007/s12010-008-8375-0. Epub 2008 Oct 4.
As technologies for utilizing biomass for fuel and chemical production continue to improve, enzymatic hydrolysis can be run at still higher solids concentrations. For hydrolyses that initially contain little or no free water (10-40% total solids, w/w), the saccharification of insoluble polymers into soluble sugars involves changes of volume, density, and proportion of insoluble solids. This poses a new challenge when determining the degree of hydrolysis (conversion yield). Experiments have shown that calculating the yield from the resulting sugar concentration in the supernatant of the slurry and using the assumed initial volume leads to significant overestimations of the yield. By measuring the proportion of insoluble solids in the slurry as well as the sugar concentration and specific gravity of the aqueous phase, it is possible to precisely calculate the degree of conversion. The discrepancies between the different ways of calculating yields are demonstrated along with a nonlaborious method for approximating yields in high solids hydrolysis.
随着将生物质用于燃料和化学品生产的技术不断改进，酶促水解可以在更高的固体浓度下进行。对于最初几乎不含有或不含有游离水（总固体含量为10 - 40%，w/w）的水解过程，将不溶性聚合物糖化转化为可溶性糖涉及体积、密度和不溶性固体比例的变化。这在确定水解程度（转化率）时带来了新的挑战。实验表明，根据浆料上清液中所得糖浓度计算产率并使用假定的初始体积会导致对产率的显著高估。通过测量浆料中不溶性固体的比例以及水相的糖浓度和比重，可以精确计算转化率。文中展示了不同产率计算方法之间的差异，并介绍了一种简便的方法来估算高固体水解中的产率。
