Mei Feng, Zhu Jiang, Guo Sheng, Zhou De-Shan, Han Juan, Yu Bin, Li Shi-Feng, Jiang Zhong-Yong, Xiong Cheng-Jie
Department of Histology and Embryology, Third Military Medical University, 400038, Chongqing, China.
Histochem Cell Biol. 2009 Jan;131(1):43-53. doi: 10.1007/s00418-008-0515-7. Epub 2008 Oct 3.
This paper aimed at investigating the alterations in interstitial cells of Cajal (ICCs) in the murine small intestine from 0-day to 56-day post-partum (P0-P56) by immunohistochemistry. The Kit+ ICCs, which were situated around myenteric nerve plexus (ICC-MY) formed a loose cellular network at P0 which changed into an intact one before P32. The density of ICC-MY increased from P0 to P12, and then decreased until P32. In contrast, the estimated total amount increased more than 15-fold at P32 than that at P0. Some Kit+/BrdU+ cells were observed at 24 h after one BrdU injection to the different-aged mice, and the number decreased from P2 to P24 and vanished at P32. Actually a few Kit+/BrdU+ cells can be observed at 1 h after one BrdU injection at P10, and the amount doubled at 24 h along with paired Kit+/BrdU+ cells. A number of BrdU+ ICCs were also labeled with CD34, CD44 and insulin-like growth factor I receptor. About 65% ICCs were BrdU+ at P32 after daily BrdU injection from P0. Our results indicate that an age-dependent proliferation is involved in the postnatal development of ICC-MY which increase greatly in cell numbers and proliferative ICCs may originate from ICCs progenitor cells.
本文旨在通过免疫组织化学方法研究产后0天至56天（P0 - P56）小鼠小肠中Cajal间质细胞（ICC）的变化。位于肌间神经丛周围的Kit⁺ ICC（ICC - MY）在P0时形成松散的细胞网络，在P32之前转变为完整的网络。ICC - MY的密度从P0增加到P12，然后下降直至P32。相比之下，P32时估计的总量比P0时增加了15倍以上。在向不同年龄小鼠注射一次BrdU后24小时观察到一些Kit⁺/BrdU⁺细胞，其数量从P2减少到P24，并在P32时消失。实际上，在P10注射一次BrdU后1小时可观察到少数Kit⁺/BrdU⁺细胞，24小时时数量翻倍并伴有成对的Kit⁺/BrdU⁺细胞。许多BrdU⁺ ICC也被CD34、CD44和胰岛素样生长因子I受体标记。从P0开始每日注射BrdU后，P32时约65%的ICC为BrdU⁺。我们的结果表明，年龄依赖性增殖参与了ICC - MY的出生后发育，其细胞数量大幅增加，增殖性ICC可能起源于ICC祖细胞。
