Zeng Rong, Zhou Zhi-wu, Wu Cui-fang, Zhou Yu-lu
Dept of Pharmacology, Hunan University of Traditional Chinese Medicine, Changsha 410007, China.
Zhongguo Zhong Yao Za Zhi. 2008 Jun;33(12):1443-5.
To determine the reversal effect of aloe emodin liposomes (AE-L) on cisplatin resistance human lung adenocarcinoma cell line A549/DDP.
The un-cytotoxic and cytotoxic concentration of AE-L, and un-cytotoxic concentration of E-L were determined by MTT. The sensitivity of cisplatin were determined by MTT assay in above 3 groups. The intracellular concentration of cisplatin was detected by inductively coupled plasma mass spectrometry (ICP-MS).
The maximum non-toxic concentration group of AE-L (2.0 mg x L(-1)) increased the sensitivity of cisplatin in A549/DDP, decreased IC50 of cisplatin in A549/DDP from 16.81 mg x L(-1) to 5.86 mg x L(-1), and the hyp-cytotoxic concentration (7.0 mg x L(-1) ) group's IC50 decreased to 4.34 mg x L(-1); AE-L groups significantly increased intracellular concentration of cisplatin in A549/DDP cells.
The results showed that aloe emodin can reverse multidrug resistance (MDR) of A549/DDP cells and the mechanism might be associated with the increase of intracellular concentration of cisplatin.
探讨芦荟大黄素脂质体（AE-L）对顺铂耐药人肺腺癌细胞系A549/DDP的逆转作用。
采用MTT法测定AE-L的无细胞毒性浓度和细胞毒性浓度，以及E-L的无细胞毒性浓度。采用MTT法检测上述3组细胞对顺铂的敏感性。采用电感耦合等离子体质谱法（ICP-MS）检测细胞内顺铂浓度。
AE-L的最大无毒浓度组（2.0mg·L⁻¹）提高了A549/DDP细胞对顺铂的敏感性，使A549/DDP细胞中顺铂的IC50从16.81mg·L⁻¹降至5.86mg·L⁻¹，低细胞毒性浓度组（7.0mg·L⁻¹）的IC50降至4.34mg·L⁻¹；AE-L组显著提高了A549/DDP细胞内顺铂浓度。
结果表明芦荟大黄素可逆转A549/DDP细胞的多药耐药性（MDR），其机制可能与细胞内顺铂浓度升高有关。
