Bonvicini Francesca, Filippone Claudia, Manaresi Elisabetta, Zerbini Marialuisa, Musiani Monica, Gallinella Giorgio
Department of Clinical and Experimental Medicine, Division of Microbiology, University of Bologna, Via Massarenti, 9I-40138 Bologna, Italy.
Virology. 2008 Nov 25;381(2):168-77. doi: 10.1016/j.virol.2008.09.002. Epub 2008 Oct 5.
Comprehension of the pathogenetic potential of human parvovirus B19 requires the definition of the complete spectrum of cellular tropism and a functional analysis of the viral genome in infected cells. In this study, we carried out a systematic functional analysis of B19 virus genome in the course of infection of susceptible bone marrow mononuclear cells and myeloblastoid UT7/EpoS1 cells, in terms of dynamics of nucleic acid synthesis. A PCR array was designed and a comprehensive analysis was performed by quantitative PCR and RT-PCR, yielding extended information on the presence and abundance of the diverse classes of viral nucleic acids, on the temporal regulation of genome expression and on its relationship with the cell cycle. The analysis performed indicate that the synthesis of viral nucleic acids is correlated to the progression through the S phase of the cell cycle, that an extended pattern of transcriptional activity occurs throughout the course of infection, with a maximal rate of transcription preceding the onset of S-phase dependent replication of the viral genome, and that utilization of transcript processing signals is relatively constant throughout the course of infection. The information obtained led to the definition of a unified model of functional and expression profiling of parvovirus B19 genome.
要理解人细小病毒B19的致病潜力，需要明确其完整的细胞嗜性谱，并对感染细胞中的病毒基因组进行功能分析。在本研究中，我们针对易感染的骨髓单核细胞和成髓细胞样UT7/EpoS1细胞在感染过程中，就核酸合成动态对B19病毒基因组进行了系统的功能分析。设计了一个PCR阵列，并通过定量PCR和RT-PCR进行了全面分析，从而获得了关于不同类别的病毒核酸的存在和丰度、基因组表达的时间调控及其与细胞周期关系的详细信息。所进行的分析表明，病毒核酸的合成与细胞周期S期的进程相关，在整个感染过程中会出现广泛的转录活性模式，在病毒基因组S期依赖性复制开始之前转录速率达到最大值，并且在整个感染过程中转录加工信号的利用相对恒定。所获得的信息促成了细小病毒B19基因组功能和表达谱统一模型的定义。
