Brown Kevin E, Liu Zhengwen, Gallinella Giorgio, Wong Susan, Mills Ian P, O'Sullivan M Gerard
Hematology Branch, National Heart, Lung, and Blood Institute, Building 10, Room CRC-3-5130, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892-1202, USA.
J Infect Dis. 2004 Dec 1;190(11):1900-7. doi: 10.1086/425420. Epub 2004 Nov 3.
Simian parvovirus (SPV) causes severe anemia in immunocompromised macaques. The closely related erythrovirus, parvovirus B19, causes anemia in susceptible humans and can be grown in human bone marrow mononuclear cells in vitro. We hypothesized that SPV may infect humans and replicate in human bone marrow mononuclear cells.
Serum samples from handlers of an SPV-seropositive macaque colony were tested by Western blot for evidence of antibodies to SPV. SPV capsid protein was expressed in insect cells, and SPV was cultured in human and macaque bone marrow mononuclear cells.
Fifty-one percent of exposed handlers (n=65) were found to be SPV seropositive, compared with 35% of nonexposed individuals (n=124). In 17% of exposed handlers, compared with 6% of nonexposed individuals, antibodies were directed to SPV but not to B19. SPV capsid proteins, like those of B19, self-assembled to form parvovirus-like particles, and these capsids, like B19 capsids, bound to globoside, suggesting that globoside is also the receptor for SPV. We demonstrated that SPV could replicate in vitro in both human and macaque bone marrow mononuclear cells and that it was cytotoxic to erythroid progenitor cells.
Our data suggest that SPV may infect human bone marrow mononuclear cells in vitro and in vivo and should be considered a potential zoonosis.
猿猴细小病毒（SPV）可导致免疫功能低下的猕猴出现严重贫血。与之密切相关的红细胞病毒——细小病毒B19，可使易感人群发生贫血，且能在人骨髓单个核细胞中体外培养。我们推测SPV可能感染人类并在人骨髓单个核细胞中复制。
采用蛋白质印迹法检测来自SPV血清阳性猕猴群落饲养员的血清样本，以寻找抗SPV抗体的证据。SPV衣壳蛋白在昆虫细胞中表达，SPV在人及猕猴骨髓单个核细胞中培养。
发现51%的暴露饲养员（n = 65）SPV血清呈阳性，相比之下，未暴露个体（n = 124）的这一比例为35%。在17%的暴露饲养员中，与6%的未暴露个体相比，抗体针对SPV而非B19。与B19的衣壳蛋白一样，SPV衣壳蛋白自行组装形成细小病毒样颗粒，并且这些衣壳与B19衣壳一样，与红细胞糖苷脂结合，表明红细胞糖苷脂也是SPV的受体。我们证明SPV能够在人及猕猴骨髓单个核细胞中体外复制，并且对红系祖细胞具有细胞毒性。
我们的数据表明，SPV可能在体外和体内感染人骨髓单个核细胞，应被视为一种潜在的人畜共患病。
