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发根农杆菌rolC肽在转基因植物中的胞质定位。

Cytosolic localization in transgenic plants of the rolC peptide from Agrobacterium rhizogenes.

作者信息

Estruch J J, Parets-Soler A, Schmülling T, Spena A

机构信息

MPI für Züchtungsforschung, Köln, Germany.

出版信息

Plant Mol Biol. 1991 Sep;17(3):547-50. doi: 10.1007/BF00040654.

DOI:10.1007/BF00040654
PMID:1884008
Abstract

The rolC gene of Agrobacterium rhizogenes codes for a peptide with an apparent molecular weight of approximately 20 kDa. Immunolocalization of the rolC peptide, in leaves of transgenic plants which are genetic mosaics for the expression of the rolC gene, is restricted to the phenotypically altered sectors. Subcellular fractionation of homogenates from 35S-rolC transgenic leaves shows the cytosolic localization of the rolC peptide.

摘要

发根农杆菌的rolC基因编码一种表观分子量约为20 kDa的肽。在rolC基因表达呈遗传嵌合状态的转基因植物叶片中，rolC肽的免疫定位仅限于表型改变的区域。对35S-rolC转基因叶片匀浆进行亚细胞分级分离，结果显示rolC肽定位于细胞质中。

相似文献

1
Cytosolic localization in transgenic plants of the rolC peptide from Agrobacterium rhizogenes.发根农杆菌rolC肽在转基因植物中的胞质定位。
Plant Mol Biol. 1991 Sep;17(3):547-50. doi: 10.1007/BF00040654.
2
Agrobacterium rhizogenes lacZ-rolC gene expression in Escherichia coli: detection of the product in transgenic plants using RolC-specific antibodies.发根农杆菌lacZ - rolC基因在大肠杆菌中的表达：利用RolC特异性抗体在转基因植物中检测产物
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3
The plant oncogene rolC is responsible for the release of cytokinins from glucoside conjugates.植物致癌基因rolC负责从糖苷共轭物中释放细胞分裂素。
EMBO J. 1991 Oct;10(10):2889-95. doi: 10.1002/j.1460-2075.1991.tb07838.x.
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The rolC promoter of Agrobacterium rhizogenes Ri plasmid is activated by sucrose in transgenic tobacco plants.发根农杆菌Ri质粒的rolC启动子在转基因烟草植株中被蔗糖激活。
Mol Gen Genet. 1994 Jul 8;244(1):15-22. doi: 10.1007/BF00280182.
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Horizontal gene transfer: regulated expression of a tobacco homologue of the Agrobacterium rhizogenes rolC gene.水平基因转移：发根农杆菌rolC基因烟草同源物的调控表达
Mol Gen Genet. 1995 Nov 27;249(3):265-73. doi: 10.1007/BF00290526.
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Constitutive or light-regulated expression of the rolC gene in transgenic potato plants has different effects on yield attributes and tuber carbohydrate composition.rolC基因在转基因马铃薯植株中的组成型表达或光调控表达对产量属性和块茎碳水化合物组成有不同影响。
Plant Mol Biol. 1993 Nov;23(4):749-57. doi: 10.1007/BF00021530.
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Restoration of fertility by antisense RNA in genetically engineered male sterile tobacco plants.通过反义RNA恢复基因工程雄性不育烟草植株的育性
Mol Gen Genet. 1993 Mar;237(3):385-94. doi: 10.1007/BF00279442.
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Cell-autonomous behavior of the rolC gene of Agrobacterium rhizogenes during leaf development: a visual assay for transposon excision in transgenic plants.发根农杆菌rolC基因在叶片发育过程中的细胞自主行为：一种用于转基因植物中转座子切除的可视化检测方法。
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Promoter of the rolC gene of Agrobacterium rhizogenes can be strongly regulated in glandular cell of transgenic tobacco.发根农杆菌rolC基因的启动子在转基因烟草的腺细胞中能够受到强烈调控。
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Auxin regulates the promoter of the root-inducing rolB gene of Agrobacterium rhizogenes in transgenic tobacco.生长素在转基因烟草中调控发根农杆菌根诱导rolB基因的启动子。
Mol Gen Genet. 1990 Aug;223(1):58-64. doi: 10.1007/BF00315797.

引用本文的文献

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Transgenic , Containing Individual Genes and Open Reading Frames Under Promoter, Exhibit Compact Habit, Reduced Plant Growth, and Altered Ethylene Tolerance in Flowers.转基因植物含有在启动子控制下的单个基因和开放阅读框，表现出紧凑的株型、生长受抑制以及花朵中乙烯耐受性改变。
Front Plant Sci. 2021 May 7;12:672023. doi: 10.3389/fpls.2021.672023. eCollection 2021.
2
Promotion of flowering and morphological alterations in Atropa belladonna transformed with a CaMV 35S-rolC chimeric gene of the Ri plasmid.用 Ri 质粒的 CaMV 35S-rolC 嵌合基因转化颠茄后对花的诱导和形态改变。
Plant Cell Rep. 1992 Dec;12(1):1-6. doi: 10.1007/BF00232412.
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本文引用的文献

1
Independent and synergistic activity of rol A, B and C loci in stimulating abnormal growth in plants.rolA、B 和 C 基因座在刺激植物异常生长中的独立和协同活性。
EMBO J. 1987 Dec 20;6(13):3891-9. doi: 10.1002/j.1460-2075.1987.tb02729.x.
2
Single genes from Agrobacterium rhizogenes influence plant development.发根农杆菌的单个基因会影响植物发育。
EMBO J. 1988 Sep;7(9):2621-9. doi: 10.1002/j.1460-2075.1988.tb03114.x.
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A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.
Bacterial plant oncogenes: the rol genes' saga.
细菌植物致癌基因：rol基因的传奇故事
Genetica. 1994;94(2-3):203-11. doi: 10.1007/BF01443434.
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Molecular genetics of auxin and cytokinin.生长素与细胞分裂素的分子遗传学
Plant Mol Biol. 1994 Dec;26(5):1499-519. doi: 10.1007/BF00016487.
一种在蛋白质免疫印迹法中检测碱性磷酸酶偶联抗抗体的快速、灵敏方法。
Anal Biochem. 1984 Jan;136(1):175-9. doi: 10.1016/0003-2697(84)90320-8.
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Molecular and genetic analysis of the transferred DNA regions of the root-inducing plasmid of Agrobacterium rhizogenes.发根农杆菌致根质粒转移DNA区域的分子与遗传分析
J Bacteriol. 1985 Oct;164(1):33-44. doi: 10.1128/jb.164.1.33-44.1985.
5
Nucleotide sequence analysis of TL-DNA of Agrobacterium rhizogenes agropine type plasmid. Identification of open reading frames.发根农杆菌章鱼碱型质粒TL-DNA的核苷酸序列分析。开放阅读框的鉴定。
J Biol Chem. 1986 Jan 5;261(1):108-21.
6
Cell-autonomous behavior of the rolC gene of Agrobacterium rhizogenes during leaf development: a visual assay for transposon excision in transgenic plants.发根农杆菌rolC基因在叶片发育过程中的细胞自主行为：一种用于转基因植物中转座子切除的可视化检测方法。
Plant Cell. 1989 Dec;1(12):1157-64. doi: 10.1105/tpc.1.12.1157.