Gene Experiment Center, University of Tsukuba, Tsukuba-shi, 305, Ibaraki, Japan.
Plant Cell Rep. 1992 Dec;12(1):1-6. doi: 10.1007/BF00232412.
Kanamycin-resistant plants of belladonna (Atropa belladonna) were obtained after Agrobacterium mediated transformation. When a rolC gene, which is one of the loci located on Ri plasmid of Agrobacterium rhizogenes, was co-introduced with a kanamycin resistant (NPT II) gene under control of a cauliflower mosaic virus 35S promoter, the rolC gene was expressed strongly in leaves, flowers, stems and roots. The transformed plants exhibited dramatic promotion of flowering, reduced apical dominance, pale and lanceolated leaves and smaller flowers. On the other hand, when native rolC gene was co-introduced with NPT II, the transgenic plants obtained did not exhibit the altered phenotypes observed in 35S-rolC transformants, and the expression level of the rolC gene was much lower than in 35S-rolC transformants. These results suggest that the morphological changes in transgenic Atropa belladonna were related to the degree of expression of the rolC gene.
颠茄（Atropa belladonna）的卡那霉素抗性植株是通过农杆菌介导的转化获得的。当 rolC 基因（位于发根农杆菌 Ri 质粒上的一个基因座之一）与受花椰菜花叶病毒 35S 启动子控制的卡那霉素抗性（NPT II）基因一起被共导入时，rolC 基因在叶片、花朵、茎和根中强烈表达。转化植株表现出明显的促进开花、降低顶端优势、叶片苍白和披针形以及花朵变小。另一方面，当将天然 rolC 基因与 NPT II 共导入时，获得的转基因植株没有表现出在 35S-rolC 转化体中观察到的改变表型，并且 rolC 基因的表达水平比在 35S-rolC 转化体中低得多。这些结果表明，转基因颠茄的形态变化与 rolC 基因的表达程度有关。
