Park H-W, Wang J H, Kim J U, Koo S-W, Kwon H J, Jang Y J
Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
Acta Anaesthesiol Scand. 2008 Sep;52(8):1046-50. doi: 10.1111/j.1399-6576.2008.01691.x.
There have been no previous studies regarding the effect of volatile anesthetics on human rhinovirus (RV) infection in airway epithelial cells of patients with an upper respiratory infection (URI). We have therefore evaluated in vitro the effect of isoflurane on RV infection in airway epithelial cells.
A549 cells and RV-infected A549 cells were treated with isoflurane for 2 or 4 h. Surface expression of intercellular adhesion molecule-1 (ICAM-1) was assessed by flow cytometry, and effects on the secretion of interleukin (IL)-6 and IL-8 were measured by ELISA. The effect on RV replication in the cells was determined by viral titer.
Isoflurane treatment for 2 or 4 h had no significant effect on ICAM-1 expression and secretion of IL-6 and IL-8 in control cells. Isoflurane also had no significant additional effect on RV-induced ICAM-1 expression and secretion of IL-6 and IL-8. Viral titers were not significantly influenced by isoflurane.
Isoflurane treatment showed no additional effects with RV on ICAM-1 expression, secretion of IL-6 and IL-8, and viral titer in A549 cells. These results suggest that isoflurane itself may not increase further RV infections, inflammations, and viral replication in patients with a viral URI.
既往尚无关于挥发性麻醉药对上呼吸道感染（URI）患者气道上皮细胞中人鼻病毒（RV）感染影响的研究。因此，我们在体外评估了异氟烷对气道上皮细胞中RV感染的影响。
将A549细胞和感染RV的A549细胞用异氟烷处理2或4小时。通过流式细胞术评估细胞间黏附分子-1（ICAM-1）的表面表达，并通过酶联免疫吸附测定法测量对白细胞介素（IL）-6和IL-8分泌的影响。通过病毒滴度确定对细胞中RV复制的影响。
异氟烷处理2或4小时对对照细胞中ICAM-1表达以及IL-6和IL-8的分泌无显著影响。异氟烷对RV诱导的ICAM-1表达以及IL-6和IL-8的分泌也无显著额外影响。病毒滴度未受到异氟烷的显著影响。
异氟烷处理对A549细胞中RV诱导的ICAM-1表达、IL-6和IL-8分泌以及病毒滴度无额外影响。这些结果表明，异氟烷本身可能不会进一步增加病毒性URI患者的RV感染、炎症和病毒复制。
