Wang Jong Hwan, Kwon Hyun-Ja, Lee Bong-Jae, Jang Yong Ju
Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
Am J Rhinol. 2007 Nov-Dec;21(6):670-4. doi: 10.2500/ajr.2007.21.3101.
Staphylococcal enterotoxin A (SEA) and staphylococcal enterotoxin B (SEB) have been reported to be important in the pathogenesis of chronic rhinosinusitis (CRS). To elucidate the pathophysiological responses in the nasal mucosae of CRS patients associated with rhinovirus (RV) infection, we investigated the effects of SEA and SEB on RV infection in A549 cells.
Changes in expression of intercellular adhesion molecule (ICAM) 1 were assessed by flow cytometry, and effects on cytokine secretion were measured by ELISA. The changes of ICAM-1, IL-1beta, IL-6, and IL-8 mRNA were assayed by real-time polymerase chain reaction. The effect of RV replication in the cells was assessed by viral culture, followed by determination of viral titer.
RV infection increased ICAM-1 expression and cytokine secretion, but the SEs did not further increase the RV-induced expression of ICAM-1, IL-1beta, IL-6, and IL-8 mRNA and protein. The SEs, however, induced dose-dependent increases in viral titer.
SEA and SEB enhanced rhinoviral replication in airway epithelial cells, indicating that airway epithelial cells with CRS are more favorable environments for RV infection.
据报道，葡萄球菌肠毒素A（SEA）和葡萄球菌肠毒素B（SEB）在慢性鼻-鼻窦炎（CRS）的发病机制中起重要作用。为了阐明CRS患者鼻黏膜与鼻病毒（RV）感染相关的病理生理反应，我们研究了SEA和SEB对A549细胞中RV感染的影响。
通过流式细胞术评估细胞间黏附分子（ICAM）1表达的变化，通过酶联免疫吸附测定法测量对细胞因子分泌的影响。通过实时聚合酶链反应检测ICAM-1、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和白细胞介素-8（IL-8）mRNA的变化。通过病毒培养评估RV在细胞中的复制效果，随后测定病毒滴度。
RV感染增加了ICAM-1表达和细胞因子分泌，但SEA和SEB并未进一步增加RV诱导的ICAM-1、IL-1β、IL-6和IL-8 mRNA及蛋白的表达。然而，SEA和SEB诱导病毒滴度呈剂量依赖性增加。
SEA和SEB增强了气道上皮细胞中鼻病毒的复制，表明CRS患者的气道上皮细胞是更有利于RV感染的环境。
