Onuma Mamoru, Kondo Hirotaka, Ono Sadaharu, Shibuya Hisashi, Sato Tsuneo
Onuma Animal Hospital, Saitama, Japan.
J Vet Med Sci. 2008 Sep;70(9):893-8. doi: 10.1292/jvms.70.893.
Twenty ferrets with histopathologically diagnosed lymphoma were classified cytomorphologically and immunohistochemically. According to site of origin, multicentric, gastrointestinal, mediastinal and cutaneous lymphomas accounted for 8 (40%), 9 (45%), 2 (10%) and 1 case (5%), respectively. According to the National Cancer Institute Working Formulation (NCI-WF), low-, high- and intermediate-grade lymphomas accounted for 4 (20%), 4 (20%) and 12 cases (60%), respectively. The 4 low-grade lymphomas showed no mitotic figures, whereas all 4 high-grade lymphomas exhibited > or = 3 mitotic figures (median,6). Higher grade thus appears to be associated with a higher number of mitotic figures. Immunohistochemical examination of 18 specimens, excluding 2 insufficient specimens, showed that 16 (88.9%) and 2 (11.1%) lymphomas were of T-cell origin and B-cell origin, respectively. According to the combination of the NCI-WF and immunophenotypes, all 4 low-grade lymphomas (2 multicentric, 1 gastrointestinal, and 1 cutaneous lymphoma) were classified as diffuse small lymphocytic lymphoma of T-cell origin. Of the 12 intermediate-grade lymphomas (6 multicentric, 4 gastrointestinal, and 2 mediastinal lymphomas), 11 were classified as diffuse mixed-cell lymphoma, and 1 as diffuse large cell lymphoma. Of these 11 lymphomas, 2 (both multicentric) were of B-cell origin, 7 (3 multicentric, 3 gastrointestinal, 1 mediastinal) were of T-cell origin, and 2 (1 multicentric, 1 mediastinal) were of unknown cell origin. The remaining 1 lymphoma (gastrointestinal) was of T-cell origin. All 4 high-grade lymphomas (gastrointestinal) were classified as diffuse immunoblastic lymphoma of T-cell origin.
对20只经组织病理学诊断为淋巴瘤的雪貂进行了细胞形态学和免疫组织化学分类。根据起源部位，多中心性、胃肠道、纵隔和皮肤淋巴瘤分别占8只（40%）、9只（45%）、2只（10%）和1只（5%）。根据美国国立癌症研究所工作分类法（NCI-WF），低级别、高级别和中级别淋巴瘤分别占4只（20%）、4只（20%）和12只（60%）。4只低级别淋巴瘤未见有丝分裂象，而所有4只高级别淋巴瘤均显示≥3个有丝分裂象（中位数为6）。因此，级别越高似乎与有丝分裂象数量越多相关。对18份标本（不包括2份标本量不足的标本）进行免疫组织化学检查显示，16只（88.9%）淋巴瘤为T细胞起源，2只（11.1%）为B细胞起源。根据NCI-WF和免疫表型的组合，所有4只低级别淋巴瘤（2只多中心性、1只胃肠道和1只皮肤淋巴瘤）均被分类为T细胞起源的弥漫性小淋巴细胞淋巴瘤。在12只中级别淋巴瘤（6只多中心性、4只胃肠道和2只纵隔淋巴瘤）中，11只被分类为弥漫性混合细胞淋巴瘤，1只被分类为弥漫性大细胞淋巴瘤。在这11只淋巴瘤中，2只（均为多中心性）为B细胞起源，7只（3只多中心性、3只胃肠道、1只纵隔）为T细胞起源，2只（1只多中心性、1只纵隔）细胞起源不明。其余1只淋巴瘤（胃肠道）为T细胞起源。所有4只高级别淋巴瘤（胃肠道）均被分类为T细胞起源的弥漫性免疫母细胞淋巴瘤。
