Terayama Yoshimi, Kato Hirotomo, Gomez Eduardo A, Uezato Hiroshi, Calvopiña Manuel, Iwata Hiroyuki, Hashiguchi Yoshihisa
Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan.
J Vet Med Sci. 2008 Sep;70(9):907-13. doi: 10.1292/jvms.70.907.
Surveillance of the distribution of sand fly species is important for prediction of the risk and expansion of Leishmania infection in endemic and surrounding areas. In the present study, a simple and reliable method of typing New World Lutzomyia species circulating in endemic areas in Ecuador was established by using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) technique. PCR-RFLP of 18S ribosomal RNA (rRNA) genes with the restriction enzyme AfaI and subsequently HinfI successfully identified seven sand fly species in nine endemic areas in Ecuador. Although intraspecific genetic-diversity affecting the RFLP-patterns was detected in a species, the patterns were species specific. The method promises to be a powerful tool for the classification of New World Lutzomyia species.
监测白蛉种类的分布对于预测利什曼原虫感染在流行地区及周边地区的风险和扩散至关重要。在本研究中，通过使用聚合酶链反应（PCR）-限制性片段长度多态性（RFLP）技术，建立了一种简单可靠的方法来鉴定在厄瓜多尔流行地区传播的新大陆罗蛉属种类。用限制性内切酶AfaI和随后的HinfI对18S核糖体RNA（rRNA）基因进行PCR-RFLP分析，成功鉴定出了厄瓜多尔9个流行地区的7种白蛉。尽管在一个物种中检测到了影响RFLP模式的种内遗传多样性，但这些模式具有物种特异性。该方法有望成为新大陆罗蛉属种类分类的有力工具。
