Al-Dakhil Abeer A, Al-Ajmi Reem A, Siddiqi Nikhat J, Ayaad Tahany H
Department of Zoology, College of Science, King Saud University, Riyadh 11495, Saudi Arabia.
Department of Biochemistry, College of Science, King Saud University, Riyadh 11495, Saudi Arabia.
Saudi J Biol Sci. 2017 Nov;24(7):1697-1703. doi: 10.1016/j.sjbs.2016.01.014. Epub 2016 Jan 19.
Studies on the distribution of sand flies are important for the control of leishmaniasis in endemic and neighboring areas. In the present study polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) was used to identify the distribution of sand flies in Al-Madinah and Asir Regions of Saudi Arabia using PCR-RFLP of 18S ribosomal RNA gene. Based on the morphological characteristics, the sand flies were differentiated into seven species viz., , , , , , PCR-RFLP of 18S ribosomal RNA (rRNA) genes with eight different restriction enzymes resulted in species-specific agarose gel electrophoresis banding patterns. Of the eight restriction enzymes used, not a single restriction enzyme by itself could separate species belonging to the same genera (like and by I) as well as those belonging to different genera (like and by I). We therefore conclude that the genetic diversity within sand fly species based on PCR-RFLP technique was nonspecific. Studies are in progress to study the viability of alternate techniques like low-stringency single specific primer polymerase chain reaction which can be used for molecular typing.
白蛉分布的研究对于控制流行地区及周边地区的利什曼病至关重要。在本研究中，利用18S核糖体RNA基因的聚合酶链反应（PCR）-限制性片段长度多态性（RFLP）技术，对沙特阿拉伯麦地那和阿西尔地区的白蛉分布进行了鉴定。根据形态学特征，白蛉被分为七个物种，即……利用八种不同的限制性内切酶对18S核糖体RNA（rRNA）基因进行PCR-RFLP分析，得到了物种特异性的琼脂糖凝胶电泳条带模式。在所使用的八种限制性内切酶中，没有一种单独的限制性内切酶能够区分属于同一属的物种（如I区分……和……）以及属于不同属的物种（如I区分……和……）。因此，我们得出结论，基于PCR-RFLP技术的白蛉物种内遗传多样性是非特异性的。目前正在进行研究，以探讨低严谨度单特异性引物聚合酶链反应等替代技术用于分子分型的可行性。
