Wang Lin, Mitra Raka M, Hasselmann Keegan D, Sato Masanao, Lenarz-Wyatt Lisa, Cohen Jerry D, Katagiri Fumiaki, Glazebrook Jane
Department of Plant Biology, Microbial and Plant Genomics Institute, University of Minnesota, 1445 Gortner Avenue, St. Paul 55108, USA.
Mol Plant Microbe Interact. 2008 Nov;21(11):1408-20. doi: 10.1094/MPMI-21-11-1408.
Expression profiling of wild-type plants and mutants with defects in key components of the defense signaling network was used to model the Arabidopsis network 24 h after infection by Pseudomonas syringae pv. maculicola ES4326. Results using the Affymetrix ATH1 array revealed that expression levels of most pathogen-responsive genes were affected by mutations in coi1, ein2, npr1, pad4, or sid2. These five mutations defined a small number of different expression patterns displayed by the majority of pathogen-responsive genes. P. syringae pv. tomato strain DC3000 elicited a much weaker salicylic acid (SA) response than ES4326. Additional mutants were profiled using a custom array. Profiles of pbs3 and ndr1 revealed major effects of these mutations and allowed PBS3 and NDR1 to be placed between the EDS1/PAD4 node and the SA synthesis node in the defense network. Comparison of coi1, dde2, and jar1 profiles showed that many genes were affected by coi1 but very few were affected by dde2 or jar1. Profiles of coi1 plants infected with ES4326 were very similar to those of wild-type plants infected with bacteria unable to produce the phytotoxin coronatine, indicating that, essentially, all COI1-dependent gene expression changes in this system are caused by coronatine.
利用野生型植物以及防御信号网络关键组分存在缺陷的突变体的表达谱，对丁香假单胞菌番茄致病变种ES4326感染24小时后的拟南芥网络进行建模。使用Affymetrix ATH1芯片得到的结果显示，大多数病原体响应基因的表达水平受到coi1、ein2、npr1、pad4或sid2突变的影响。这五个突变定义了大多数病原体响应基因所呈现的少数几种不同表达模式。丁香假单胞菌番茄致病变种DC3000引发的水杨酸（SA）反应比ES4326弱得多。使用定制芯片对其他突变体进行了分析。pbs3和ndr1的分析结果揭示了这些突变的主要影响，并使PBS3和NDR1能够定位在防御网络中EDS1/PAD4节点和SA合成节点之间。coi1、dde2和jar1分析结果的比较表明，许多基因受coi1影响，但受dde2或jar1影响的基因很少。用ES4326感染的coi1植物的分析结果与用不能产生植物毒素冠菌素的细菌感染的野生型植物的分析结果非常相似，这表明在这个系统中，基本上所有依赖COI1的基因表达变化都是由冠菌素引起的。
