Xu Peipei, Chen Haiying, Hu Jinbo, Pang Xiaocheng, Jin Jing, Cai Weiming
Laboratory of Photosynthesis and Environment, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China.
University of Chinese Academy of Sciences, Beijing 100039, China.
iScience. 2022 Apr 29;25(5):104331. doi: 10.1016/j.isci.2022.104331. eCollection 2022 May 20.
Pectin is biosynthesized in a highly methylated form and is partially de-methylated by pectin methylesterase (PME) activity. Plant PMEs play a critical role in cell wall remodeling in many physiological processes. Here, we studied Arabidopsis seedlings, which had been exposed to simulated or actual microgravity. Simulated microgravity inhibited total PME activity in Arabidopsis seedlings. We identified that expression played a major role in the microgravity-induced inhibition of PME activity. mutants did not exhibit the enlarged leaf area of Arabidopsis seedlings observed under spaceflight microgravity. The downregulation of expression in response to microgravity was due, in part, to changes in methylation patterns. The sexual offspring of the plants grown during spaceflight retained the methylation changes at locus for one generation and thus contribute to the physiological adaptation to microgravity among F offspring seed generation. We conclude that contributes to the spaceflight-induced transgenerational responses in Arabidopsis.
果胶以高度甲基化的形式生物合成，并通过果胶甲酯酶（PME）的活性进行部分去甲基化。植物PME在许多生理过程的细胞壁重塑中起关键作用。在这里，我们研究了暴露于模拟或实际微重力下的拟南芥幼苗。模拟微重力抑制了拟南芥幼苗中的总PME活性。我们确定， 表达在微重力诱导的PME活性抑制中起主要作用。 突变体没有表现出在太空飞行微重力下观察到的拟南芥幼苗叶片面积增大的现象。响应微重力的 表达下调部分是由于甲基化模式的变化。在太空飞行期间生长的植物的有性后代在 位点保留甲基化变化一代，从而有助于F后代种子世代对微重力的生理适应。我们得出结论， 在拟南芥中促成了太空飞行诱导的跨代反应。
