Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
Chemosphere. 2008 Nov;73(9):1387-92. doi: 10.1016/j.chemosphere.2008.08.040. Epub 2008 Oct 7.
A new bioaugmentation method for petroleum- and salt-contaminated soil was presented, in which wheat straw was used to enhance salt leaching and subsequent petroleum degradation by a bacteria-fungi consortium of Enterobacter cloacae and Cunninghamella echinulata. The effectiveness of a coarse wheat straw layer in inhibiting capillary-induced upward salt movement and in enhancing growth of E. cloacae and C. echinulata was shown in the laboratory and a 7000-m(2) field study in Henan Province, China. In the field study, the Na(+) concentration in remediated soil at 1-25 cm depth decreased from 1597 ± 394 to 543 ± 217 mg kg(-1), while Cl(-) decreased from 1520 ± 922 to 421 ± 253 mg kg(-1). The wheat straw increased bacterial biomass by 170-fold and fungi 11-fold compared to control soil without wheat straw. The concentration of total petroleum hydrocarbons decreased from 6320 ± 1180 to 2260 ± 420 mg kg(-1) after 45 d of treatment. Wheat was cultivated on remediated soil and grain yield reached 72% of that obtained in normal farmland adjacent to the study site. The results demonstrated the effectiveness of wheat straw in enhancing bioaugmentation of the petroleum- and salt-contaminated soil and indicated a high application potential.
提出了一种新的生物强化方法，用于石油和盐分污染土壤，该方法使用小麦秸秆来增强由肠杆菌属和卷枝毛霉组成的细菌-真菌共生物对盐分淋洗和随后的石油降解作用。在实验室和中国河南省的 7000m²现场研究中，均表明粗小麦秸秆层在抑制毛细作用引起的盐分向上运移和促进肠杆菌属和卷枝毛霉生长方面的有效性。在现场研究中，修复土壤中 1-25cm 深度处的 Na+浓度从 1597±394mg/kg 降至 543±217mg/kg，而 Cl-浓度从 1520±922mg/kg 降至 421±253mg/kg。与未添加小麦秸秆的对照土壤相比，小麦秸秆使细菌生物量增加了 170 倍，真菌增加了 11 倍。处理 45 天后，总石油烃的浓度从 6320±1180mg/kg 降至 2260±420mg/kg。小麦种植在修复后的土壤上，其籽粒产量达到了与研究地点相邻的正常农田产量的 72%。结果表明，小麦秸秆在增强石油和盐分污染土壤的生物强化方面是有效的，并表明了其具有很高的应用潜力。
