Fan Li, Gong Xianhe, Lv Quanwei, Bin Denghui, Wang Li'Ao
College of Resource and Safety Engineering, Chongqing University, Chongqing 400044, China.
Chongqing Academy of Ecology and Environmental Sciences, Chongqing 401336, China.
Microorganisms. 2024 Feb 2;12(2):318. doi: 10.3390/microorganisms12020318.
Oil-based drilling cuttings (OBDCs) contain petroleum hydrocarbons with complex compositions and high concentrations, which have highly carcinogenic, teratogenic, and mutagenic properties. In this study, three highly efficient petroleum hydrocarbon-degrading bacteria were screened from OBDCs of different shale gas wells in Chongqing, China, and identified as sp. and sp. Because of their ability to degrade hydrocarbons of various chain lengths, a new method was proposed for degrading petroleum hydrocarbons in shale gas OBDCs by combining different bacterial species. Results showed that the bacterial consortium, consisting of the three strains, exhibited the highest degradation rate for petroleum hydrocarbons, capable of degrading 74.38% of long-chain alkanes and 93.57% of short-chain alkanes, respectively. Moreover, the petroleum hydrocarbon degradation performance of the bacterial consortium in actual OBDCs could reach 90.60% in the optimal conditions, and the degradation kinetic process followed a first-order kinetic model. This study provides a certain technical reserve for the bioremediation of shale gas OBDCs.
油基钻井岩屑（OBDCs）含有成分复杂且浓度高的石油烃，具有高度致癌、致畸和致突变特性。在本研究中，从中国重庆不同页岩气井的油基钻井岩屑中筛选出三株高效石油烃降解菌，鉴定为 菌属和 菌属。由于它们能够降解各种链长的烃类，因此提出了一种通过组合不同细菌种类来降解页岩气油基钻井岩屑中石油烃的新方法。结果表明，由这三株菌株组成的细菌联合体对石油烃的降解率最高，分别能够降解74.38%的长链烷烃和93.57%的短链烷烃。此外，在最佳条件下，细菌联合体在实际油基钻井岩屑中的石油烃降解性能可达90.60%，降解动力学过程遵循一级动力学模型。本研究为页岩气油基钻井岩屑的生物修复提供了一定的技术储备。
