Kageyama Akiko, Haga Tomomi, Kasai Hiroaki, Shizuri Yoshikazu, Omura Satoshi, Takahashi Yoko
Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan.
Int J Syst Evol Microbiol. 2008 Oct;58(Pt 10):2429-32. doi: 10.1099/ijs.0.65210-0.
Strain HG667(T), isolated from surface seawater collected at the Kesennuma ferry port in Miyagi Prefecture, Japan, was found to be a Gram-positive, catalase-positive bacterium comprising irregular short rods and cocci. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major menaquinone was MK-8(H4). Mycolic acids were not detected. The G+C content of the DNA was 70 mol%. Analysis of the 16S rRNA gene sequence revealed that the strain represents a novel lineage within the family Intrasporangiaceae, order Actinomycetales, being associated with the genus Kribbia. On the basis of morphological, biochemical and chemotaxonomic properties of the strain, together with phylogenetic data relating to the 16S rRNA gene sequence, HG667(T) represents a novel genus and species in the family Intrasporangiaceae, for which the name Marihabitans asiaticum gen. nov., sp. nov. is proposed. The type strain of Marihabitans asiaticum is HG667(T) (=MBIC07497(T) =DSM 18935(T)).
从日本宫城县气仙沼渡轮码头采集的表层海水中分离出的菌株HG667(T)，被发现是一种革兰氏阳性、过氧化氢酶阳性细菌，由不规则短杆状和球状细胞组成。细胞壁肽聚糖中的诊断性二氨基酸是内消旋二氨基庚二酸。主要甲基萘醌是MK-8(H4)。未检测到分枝菌酸。DNA的G+C含量为70 mol%。对16S rRNA基因序列的分析表明，该菌株代表放线菌目内孢囊菌科中的一个新谱系，与克里布菌属相关。基于该菌株的形态、生化和化学分类学特性，以及与16S rRNA基因序列相关的系统发育数据，HG667(T)代表内孢囊菌科中的一个新属和新种，为此提出新属名亚洲海栖菌属（Marihabitans）、新种名亚洲海栖菌（Marihabitans asiaticum）。亚洲海栖菌的模式菌株是HG667(T)（=MBIC07497(T)=DSM 18935(T)）。
