Kageyama Akiko, Matsumoto Atsuko, Omura Satoshi, Takahashi Yoko
Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan.
Int J Syst Evol Microbiol. 2008 Jul;58(Pt 7):1547-51. doi: 10.1099/ijs.0.65042-0.
Strain KV-663(T) was isolated from a soil sample collected from a paddy field in Japan by using GPM agar plates. Strain YM21-029 was obtained from a lake sediment sample by using HSV agar. The two novel strains were Gram-positive, catalase-positive, rod-shaped bacteria with ll-diaminopimelic acid as the diagnostic diamino acid of the cell-wall peptidoglycan. The major menaquinone was MK-8(H(4)). Mycolic acids were not detected. The G+C content of the DNA was 69-70 mol%. 16S rRNA gene sequence analysis revealed that these strains represent a novel lineage within the family Intrasporangiaceae, order Actinomycetales, and are related to members of the genera Intrasporangium and Terracoccus. Based on morphological, biochemical and chemotaxonomic properties, together with phylogenetic analysis based on 16S rRNA gene sequences, the two new strains are considered to represent a novel species of a new genus, for which the name Humibacillus xanthopallidus gen. nov., sp. nov. is proposed. The type strain of the type species, Humibacillus xanthopallidus, is KV-663(T) (=NRRL B-24471(T)=NBRC 101803(T)).
菌株KV-663(T)是通过使用GPM琼脂平板从日本稻田采集的土壤样本中分离得到的。菌株YM21-029是通过使用HSV琼脂从湖泊沉积物样本中获得的。这两种新菌株为革兰氏阳性、过氧化氢酶阳性、杆状细菌，其细胞壁肽聚糖的诊断性二氨基酸为ll-二氨基庚二酸。主要的甲基萘醌是MK-8(H(4))。未检测到分枝菌酸。DNA的G+C含量为69-70 mol%。16S rRNA基因序列分析表明，这些菌株代表放线菌目内孢囊菌科中的一个新谱系，并且与内孢囊菌属和地球菌属的成员相关。基于形态学、生化和化学分类学特性，以及基于16S rRNA基因序列的系统发育分析，这两个新菌株被认为代表一个新属的一个新物种，为此提出了黄苍白土芽孢杆菌（Humibacillus xanthopallidus）的新属名和新种名。模式种黄苍白土芽孢杆菌的模式菌株是KV-663(T)（=NRRL B-24471(T)=NBRC 101803(T)）。
