Schmeiser H H, Scherf H R, Wiessler M
Institute of Toxicology and Chemotherapy, German Cancer Research Center, Heidelberg.
Cancer Lett. 1991 Aug;59(2):139-43. doi: 10.1016/0304-3835(91)90178-k.
The plant extract aristolochic acid, which consists mainly of aristolochic acid I (AAI) and aristolochic acid II (AAII), induces tumors in rats and mice. Thin-tissue sections of rat tumors induced by AAI and of mouse tumors induced by aristolochic acid, were analyzed for c-Ha-ras mutations in codon 61. Areas of neoplastic and histologically normal tissue were manually scraped out and separated. Using the polymerase chain reaction (PCR) and mutation detection by selective oligonucleotide hybridization, we observed AT----TA transversion mutations in DNA of neoplastic portions, but not in DNA of adjacent normal tissue in both rat and mouse tumors.
主要由马兜铃酸I（AAI）和马兜铃酸II（AAII）组成的植物提取物马兜铃酸可在大鼠和小鼠体内诱发肿瘤。对由AAI诱发的大鼠肿瘤组织切片以及由马兜铃酸诱发的小鼠肿瘤组织切片，分析了密码子61处的c-Ha-ras突变情况。手动刮取并分离肿瘤性组织区域和组织学上正常的组织区域。通过聚合酶链反应（PCR）和选择性寡核苷酸杂交检测突变，我们在大鼠和小鼠肿瘤的肿瘤部分DNA中均观察到了AT----TA颠换突变，而在相邻正常组织的DNA中未观察到这种突变。
