Koenig Samuel, Savage Candida, Kim Jonathan P
Department of Marine Science, University of Otago, 310 Castle Street, Dunedin 9016, New Zealand.
Mar Pollut Bull. 2008 Dec;56(12):2003-8. doi: 10.1016/j.marpolbul.2008.08.010. Epub 2008 Oct 8.
The detection of urinary polycyclic aromatic hydrocarbon (PAH) metabolites by fluorescence spectrophotometry is particularly effective as a practical means to assess PAH exposure in decapod crabs. However, the practical application of this technique has thus far only been tested for the European shore crab (Carcinus maenas) and only a few field studies have been conducted in heavily polluted areas. The present study evaluated the adaptability of this method as a rapid, cost-effective and non-destructive biomonitoring tool for the New Zealand crab species, Macrophthalmus hirtipes (stalk-eyed mud crab). A field gradient could be detected among the sites and different input sources of PAH contamination could be discerned through the differentiation of pyrogenic and petrogenic PAH signatures. The present study shows that the fluorescence screening method is sensitive to relatively low levels of PAH contamination and more broadly applicable to smaller crab species than C. maenas, for which the technique was developed.
通过荧光分光光度法检测十足目蟹类尿液中的多环芳烃（PAH）代谢物，作为评估PAH暴露的一种实用手段，特别有效。然而，迄今为止，该技术的实际应用仅在欧洲岸蟹（Carcinus maenas）上进行了测试，并且仅在污染严重的地区进行了少数实地研究。本研究评估了该方法作为一种快速、经济高效且无损的生物监测工具对新西兰蟹种——粗糙巨眼蟹（Macrophthalmus hirtipes，柄眼泥蟹）的适用性。通过该方法能够在不同地点检测到PAH污染的现场梯度，并通过区分热解源和石油源PAH特征来识别不同的PAH污染输入源。本研究表明，荧光筛选方法对相对较低水平的PAH污染敏感，并且比该技术所针对的欧洲岸蟹更广泛地适用于较小的蟹种。
