Liu Tong, Zhang Jian
Department of Dermatology, First Affiliated Hospital, Medical School, Xi'an Jiaotong University, Xi'an 710061, China.
J Huazhong Univ Sci Technolog Med Sci. 2008 Oct;28(5):599-603. doi: 10.1007/s11596-008-0525-7. Epub 2008 Oct 10.
This study investigated the contents and distribution of collagen V (Col V) in skin lesions of the patients with systemic sclerosis (SSc) and its roles in the pathogenesis. The contents and distribution for alpha1 chain of collagen type I, III and V [alpha1 (I), alpha1 (III) and alpha1 (V)] in skin lesions of 36 patients with SSc (9 cases of mild fibrosis, 14 moderate, and 13 severe) were detected by using immunohistochemical SP method. Six cases of normal skin tissues served as controls. The results showed that there was diffuse distribution for three kinds of collagens in dermis. The deep staining alpha1 (I) and alpha1 (III) masses or bands were seen in reticular layer, while alpha1 (V) was distributed more homogeneously. From control to weak, moderate and severe fibrosis stages, alpha1 (I), alpha1 (III) and alpha1 (V) showed a gradually increased trend in skin lesions (P<0.05). alpha1 (V) was obviously elevated in skin lesions at early stage and persisted in whole fibrotic process and risen in greater contents, while alpha1 (I) and alpha1 (III) were to go higher late and were apparently elevated at moderate and late stages. Compared with alpha1 (I), alpha1 (V) took leading increase at early stage in skin lesions (P<0.01), and had more elevated contents than alpha1 (III) at moderate and late stages. The fibrotic changes in dermal reticular layer occurred earlier than those in papillary layer, and the abnormalities of alpha1 (V)/alpha1 (I) ratio appeared before alpha1 (III)/alpha1 (I) ratio. It was concluded that a lot of alpha1 (V) began to deposit in greater contents prior to alpha1 (I) and alpha1 (III) at early stage in SSc and persisted in whole fibrotic process. The changes of alpha1 (V) contents in reticular layer occurred earlier than those in papillary layer, and it suggested that the fibrosis in reticular layer appeared earlier.
本研究调查了系统性硬化症（SSc）患者皮肤病变中Ⅴ型胶原蛋白（Col V）的含量和分布及其在发病机制中的作用。采用免疫组织化学SP法检测36例SSc患者（轻度纤维化9例、中度14例、重度13例）皮肤病变中Ⅰ型、Ⅲ型和Ⅴ型胶原蛋白α1链[α1（Ⅰ）、α1（Ⅲ）和α1（Ⅴ）]的含量和分布。选取6例正常皮肤组织作为对照。结果显示，三种胶原蛋白在真皮中呈弥漫性分布。在网状层可见深染的α1（Ⅰ）和α1（Ⅲ）团块或条带，而α1（Ⅴ）分布更均匀。从对照到轻度、中度和重度纤维化阶段，皮肤病变中α1（Ⅰ）、α1（Ⅲ）和α1（Ⅴ）呈逐渐升高趋势（P<0.05）。α1（Ⅴ）在皮肤病变早期明显升高，并在整个纤维化过程中持续存在且含量升高幅度更大，而α1（Ⅰ）和α1（Ⅲ）后期升高，在中度和晚期明显升高。与α1（Ⅰ）相比，α1（Ⅴ）在皮肤病变早期率先升高（P<0.01），在中度和晚期其含量比α1（Ⅲ）升高更明显。真皮网状层的纤维化改变早于乳头层，α1（Ⅴ）/α1（Ⅰ）比值异常早于α1（Ⅲ）/α1（Ⅰ）比值。结论为，在SSc早期，大量α1（Ⅴ）开始比α1（Ⅰ）和α1（Ⅲ）以更高含量沉积，并在整个纤维化过程中持续存在。网状层α1（Ⅴ）含量变化早于乳头层，提示网状层纤维化出现更早。
