Research & Development, Healthpoint Ltd, Fort Worth, TX 76107, USA.
Int Wound J. 2010 Apr;7(2):87-95. doi: 10.1111/j.1742-481X.2010.00659.x.
Clostridium collagenase has been widely used in biomedical research to dissociate tissues and isolate cells; and, since 1965, as a therapeutic drug for the removal of necrotic wound tissues. Previous studies found that purified collagenase-treated extracellular matrix stimulated cellular response to injury and increased cell proliferation and migration. This article presents an in vitro study investigating the digestive ability of Clostridium collagenase on human collagen types I, III, IV, V and VI. Our results showed that Clostridium collagenase displays proteolytic power to digest all these types of human collagen, except type VI. The degradation products derived were tested in cell migration assays using human keratinocytes (gold surface migration assay) and fibroblasts (chemotaxis cell migration assay). Clostridium collagenase itself and the degradation products of type I and III collagens showed an increase in keratinocyte and fibroblast migration, type IV-induced fibroblast migration only, and the remainder showed no effects compared with the control. The data indicate that Clostridium collagenase can effectively digest collagen isoforms that are present in necrotic wound tissues and suggest that collagenase treatment provides several mechanisms to enhance cell migration: collagenase itself and collagen degradation products.
梭菌胶原酶在生物医学研究中被广泛用于分离组织和分离细胞；自 1965 年以来，它一直被用作治疗坏死性伤口组织的药物。先前的研究发现，经纯化的胶原酶处理的细胞外基质可刺激细胞对损伤的反应，并增加细胞增殖和迁移。本文介绍了一项体外研究，研究了梭菌胶原酶对人胶原 I、III、IV、V 和 VI 型的消化能力。我们的结果表明，梭菌胶原酶具有蛋白水解能力，可以消化所有这些类型的人胶原，除了 VI 型。用角化细胞（金表面迁移测定法）和成纤维细胞（趋化细胞迁移测定法）进行细胞迁移测定，测试了所得的降解产物。梭菌胶原酶本身以及 I 型和 III 型胶原的降解产物增加了角化细胞和成纤维细胞的迁移，IV 型胶原仅诱导成纤维细胞的迁移，而其余与对照相比没有效果。数据表明，梭菌胶原酶可以有效地消化坏死性伤口组织中存在的胶原同工型，并表明胶原酶处理提供了几种增强细胞迁移的机制：胶原酶本身和胶原降解产物。
