Alemán Ingrist, Alex Rixio, Ramírez Margelis, Hung Alida, Ramírez Carlos
Laboratorio de Biología Molecular, Cátedra de Bioquímica, Escuela de Medicina José Maria Vargas, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela.
Invest Clin. 2008 Sep;49(3):321-30.
Preeclampsia (PE) is a disease that onsets in the second half of pregnancy. This condition is characterized by hypertension, proteinuria and, frequently, intrauterine growth restriction (IUGR). Nitric oxide (NO) regulates blood flow in the human placenta, it induces vasodilatation, inhibition of platelet aggregation and prevents adhesion of platelets to endothelial cells. In this work, nitrite levels were evaluated in the sera of peripheral blood of normal pregnant women (n = 46) and women with PE (n = 50); additionally, the expression of endothelial constitutive nitric oxide and inducible synthases (eNOS and iNOS, respectively) of placental tissues, were determined. An increased concentration of serum nitrites from patients with PE, in relation to normal pregnant women (150.64 +/- 8.94 vs 40.62 +/- 1.65 microM, p < 0.00001) was observed. An increased expression of nitric oxide synthases (eNOS and iNOS), in the placental tissues of (PE) patients, as compared to that of normal pregnant women (iNOS 4.29 +/- 1.51 vs 0.59 +/- 0.13; eNOS 1.78 +/- 0.74 vs 0.46 +/- 0.22, p < 0.005) was also observed. Our results show that there exists a relationship between serum nitrites concentration and the expression of eNOS and iNOS, as analyzed in protein extracts of placental tissues.
子痫前期（PE）是一种在妊娠后半期发病的疾病。这种病症的特征为高血压、蛋白尿，且常常伴有胎儿生长受限（IUGR）。一氧化氮（NO）调节人类胎盘的血流，它可诱导血管舒张、抑制血小板聚集并防止血小板黏附于内皮细胞。在本研究中，评估了正常孕妇（n = 46）和患有子痫前期的孕妇（n = 50）外周血血清中的亚硝酸盐水平；此外，还测定了胎盘组织中内皮型一氧化氮合酶和诱导型一氧化氮合酶（分别为eNOS和iNOS）的表达。观察到子痫前期患者血清亚硝酸盐浓度相对于正常孕妇有所升高（150.64±8.94对40.62±1.65微摩尔，p < 0.00001）。与正常孕妇相比，子痫前期患者胎盘组织中一氧化氮合酶（eNOS和iNOS）的表达也有所增加（iNOS 4.29±1.51对0.59±0.13；eNOS 1.78±0.74对0.46±0.22，p < 0.005）。我们的结果表明，如在胎盘组织蛋白提取物中所分析的，血清亚硝酸盐浓度与eNOS和iNOS的表达之间存在关联。
