Schiessl Barbara, Mylonas Ioannis, Kuhn Christina, Kunze Susanne, Schulze Sandra, Friese Klaus, Jeschke Udo
1st Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University Munich, Maistrasse, Munich, Germany.
Arch Med Res. 2006 Nov;37(8):967-75. doi: 10.1016/j.arcmed.2006.03.011.
Nitric oxide seems to play important roles in the physiology of placental blood circulation, although their expression in pathological placentas and their role is still unclear. Therefore, the aim of this study was to investigate the expression of estrogen receptor alpha (ERalpha), estrogen receptor beta (ERbeta) and the endothelial NO synthase (eNOS) and inducible NO synthase (iNOS) in intrauterine growth-restricted (IUGR) placentas, hemolysis, elevated liver enzymes, low platelets (HELLP) placentas and in normal healthy control placentas.
Slides of paraffin-embedded placental tissue were obtained after delivery from patients diagnosed with IUGR, HELLP and normal term placentas and analyzed for eNOS and iNOS, as well as ERalpha/beta expression. Intensity of immunohistochemical reaction was analyzed using a semi-quantitative score, and statistical analysis was performed. In addition, Western blot experiments were performed for comparison of staining intensities obtained by immunohistochemistry and Western blot.
Expression of eNOS and iNOS is significantly reduced in trophoblast cells of placentas with HELLP. However, ERbeta expression in HELLP placentas demonstrated a significantly elevated expression intensity compared to normal controls. ERalpha expression was not significantly different in all three pathologies investigated.
We speculate that the increased ERbeta expression in both syncytiotrophoblast and extravillous trophoblast cells represents accelerated proliferation of placental tissue or can be seen as a compensatory effect in HELLP placentas.
一氧化氮似乎在胎盘血液循环的生理过程中发挥重要作用，尽管其在病理性胎盘中的表达及其作用仍不清楚。因此，本研究的目的是调查雌激素受体α（ERα）、雌激素受体β（ERβ）、内皮型一氧化氮合酶（eNOS）和诱导型一氧化氮合酶（iNOS）在宫内生长受限（IUGR）胎盘、溶血、肝酶升高、血小板减少（HELLP）胎盘以及正常健康对照胎盘中的表达情况。
从诊断为IUGR、HELLP的患者以及足月正常胎盘的患者分娩后获取石蜡包埋的胎盘组织切片，分析eNOS、iNOS以及ERα/β的表达。使用半定量评分分析免疫组化反应强度，并进行统计分析。此外，进行蛋白质印迹实验以比较免疫组化和蛋白质印迹获得的染色强度。
HELLP胎盘的滋养层细胞中eNOS和iNOS的表达显著降低。然而，与正常对照相比，HELLP胎盘中ERβ的表达强度显著升高。在所研究的所有三种病理情况下，ERα的表达没有显著差异。
我们推测合体滋养层细胞和绒毛外滋养层细胞中ERβ表达的增加代表胎盘组织的加速增殖，或者可被视为HELLP胎盘中的一种代偿效应。
